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Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to asses data reproducibility

ABSTRACT: Objective Label-free quantitative proteomics has emerged as a powerful strategy to obtain high quality quantitative measures of the proteome with only a very small quantity of total protein extract. Because our research projects were requiring the application of bottom-up shotgun Mass Spectrometry proteomics in the pathogenic yeasts Candida glabrata and Candida albicans, we performed preliminary experiments to i) obtained a precise list of all the proteins for which measures of abundance could be obtain and ii) assess the reproducibility of the results arising respectively from biological and technical replicates. Data description C. glabrata and C. albicans cells were grown in minimal liquid medium. Three independent time-courses were performed simultaneously, in each species, and an alkaline pH stress was induced for two of them. Cells were collected 10 and 60 minutes after stress induction and proteins were extracted. They were analysed and quantified two time by mass spectrometry. Our final dataset thus comprises label-free quantitative proteomics results for 24 samples (two species, three time-courses, two time points and two runs of mass spectrometry). Statistical procedures were applied to identify proteins with differential abundances between stressed and unstressed situations. Considering that C. glabrata and C. albicans are human pathogens which face important pH fluctuations during a human host infection, this dataset has a potential value to other researchers in the field.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Candida Glabrata (yeast) (torulopsis Glabrata) Candida Albicans (yeast)

SUBMITTER: Thibaut LEGER

LAB HEAD: Pr. Gaëlle Lelandais

PROVIDER: PXD014125 | Pride | 2019-07-08

REPOSITORIES: pride

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			Action	DRS
	1445007-Cglabrata-QUANTI.csv	Csv
	1445007-Q1-CTRL-10-Cglabrata.pdResult	Other
	1445007-Q1-CTRL-10-Cglabrata.raw	Raw
	1445007-Q1-CTRL-10-Cglabrata.xlsx	Xlsx
	1445007-Q10-CTRL-60-Cglabrata.pdResult	Other

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Label-free quantitative proteomics in Candida yeast species: technical and biological replicates to asses data reproducibility

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