Dataset Information

Re-expression of SMARCA4/BRG1 in Small Cell Carcinoma of Ovary, Hypercalcemic Type (SCCOHT) promotes an epithelial-like gene signature through an AP-1-dependent mechanism

ABSTRACT: Small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) is a rare and aggressive form of ovarian cancer. SCCOHT tumors have inactivating mutations in SMARCA4 (BRG1), one of the two mutually exclusive ATPases of the SWI/SNF chromatin remodeling complex. To address the role that BRG1 loss plays in SCCOHT tumorigenesis, we performed integrative multi-omic analyses in SCCOHT cell lines +/- BRG1 re-expression. BRG1 re-expression induced a gene and protein signature similar to an epithelial cell and gained chromatin accessibility sites correlated with other epithelial originating TCGA tumors. Furthermore, AP-1 motifs were enriched at the promoters of highly upregulated epithelial genes. Using a dominant negative AP-1 cell line, we found that both AP-1 DNA binding activity and BRG1 re-expression are necessary for the gene and protein expression of epithelial genes. Our study demonstrates that BRG1 re-expression drives an epithelial-like gene and protein signature in SCCOHT cells that depends upon AP-1 activity.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Gian Luca Negri

LAB HEAD: Gregg Morin

PROVIDER: PXD014134 | Pride | 2021-01-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	sc_04Apr2018_YW_BRG1_TMT11_1.raw	Raw
	sc_04Apr2018_YW_BRG1_TMT11_10.raw	Raw
	sc_04Apr2018_YW_BRG1_TMT11_11.raw	Raw
	sc_04Apr2018_YW_BRG1_TMT11_12.raw	Raw
	sc_04Apr2018_YW_BRG1_TMT11_2.raw	Raw

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Publications

Re-expression of SMARCA4/BRG1 in small cell carcinoma of ovary, hypercalcemic type (SCCOHT) promotes an epithelial-like gene signature through an AP-1-dependent mechanism.

Orlando Krystal Ann KA Douglas Amber K AK Abudu Aierken A Wang Yemin Y Tessier-Cloutier Basile B Su Weiping W Peters Alec A Sherman Larry S LS Moore Rayvon R Nguyen Vinh V Negri Gian Luca GL Colborne Shane S Morin Gregg B GB Kommoss Friedrich F Lang Jessica D JD Hendricks William Pd WP Raupach Elizabeth A EA Pirrotte Patrick P Huntsman David G DG Trent Jeffrey M JM Parker Joel S JS Raab Jesse R JR Weissman Bernard E BE

eLife 20201223

Small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) is a rare and aggressive form of ovarian cancer. SCCOHT tumors have inactivating mutations in <i>SMARCA4</i> (BRG1), one of the two mutually exclusive ATPases of the SWI/SNF chromatin remodeling complex. To address the role that BRG1 loss plays in SCCOHT tumorigenesis, we performed integrative multi-omic analyses in SCCOHT cell lines +/- BRG1 reexpression. BRG1 reexpression induced a gene and protein signature similar to an epithelia ...[more]

PMID: 33355532

Dataset Information

Re-expression of SMARCA4/BRG1 in Small Cell Carcinoma of Ovary, Hypercalcemic Type (SCCOHT) promotes an epithelial-like gene signature through an AP-1-dependent mechanism

Dataset's files

Publications

Re-expression of SMARCA4/BRG1 in small cell carcinoma of ovary, hypercalcemic type (SCCOHT) promotes an epithelial-like gene signature through an AP-1-dependent mechanism.

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