Proteomics

Dataset Information

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Malus x domestica microshoot chloroplast proteome at different growth temperatures

ABSTRACT: Chloroplasts were purified by centrifugation in Percoll gradient from low temperature (4 C), control (22 C) and high temperature (30 C) grown Malus x domestica microshoots. The proteins were digested using filter aided sample processing (FASP) and analyzed using nano-LC MS/MS. Constant scan rate of 2 Hz and range from 100 to 2,200 m/z was used for the MS spectra acquisition. Data-dependent auto-MS/MS method (Compass 1.5 acquisition and processing software, Bruker Daltonics) was used for selecting the 10 most abundant precursor ions for fragmentation. The MS/MS summation time was adjusted based on precursor intensity within the range 1-10 Hz. Active exclusion of already analyzed precursors was used with a release time of 30 s. Precursor ion isolation window and the CID collision energy were adjusted between 3-9 Th and 34-55 eV as a function of the m/z value, respectively. The data files (9 low temperature samples, 12 control samples and 9 high temperature sampes) are described in the "metadata.txt" file.

INSTRUMENT(S): maXis

ORGANISM(S): Malus Domestica

SUBMITTER: Perttu Haimi  

LAB HEAD: Perttu Haimi

PROVIDER: PXD014212 | Pride | 2019-10-03

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
GeSeqJob_mf_gene_proteins.zip Other
maxquant_parameters.txt Txt
metadata.txt Txt
proteinGroups.txt Txt
s_10H_GD5_01_6638.d.zip Other
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Publications

Differential Chloroplast Proteomics of Temperature Adaptation in Apple (Malus x domestica Borkh.) Microshoots.

Morkūnaitė-Haimi Šarūnė Š   Vinskiene Jurgita J   Stanienė Gražina G   Haimi Perttu P  

Proteomics 20191001 19

Temperature stress is one of the most common external factors that plants have to adapt to. Accordingly, plants have developed several adaptation mechanisms to deal with temperature stress. Chloroplasts are one of the organelles that are responsible for the sensing of the temperature signal and triggering a response. Here, chloroplasts are purified from low temperature (4° C), control (22° C) and high temperature (30° C) grown Malus x domestica microshoots. The purity of the chloroplast fraction  ...[more]

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