Dataset Information

The global response of Pseudomonas putida to a complex lignolysate

ABSTRACT: In this work, we developed processes using ionic liquids to obtain size-defined soluble aromatic-rich streams from sorghum biomass to assess their biocompatibility with P. putida. Growth was unexpectedly higher than that observed on a main aromatic compound. Subsequently, proteomics and metabolomics analysis revealed that BCD liquor contains additional carbon sources except aromatics. Our comparative proteomic analysis first revealed significant up-regulation of a diverse set of proteins in P. putida grown in the complex lignolysate to understand their function and regulation.

INSTRUMENT(S): 6550 iFunnel Q-TOF LC/MS

ORGANISM(S): Pseudomonas Putida Kt2440

TISSUE(S): Cell Culture

SUBMITTER: Christopher Petzold

LAB HEAD: Christopher J.Petzold

PROVIDER: PXD014285 | Pride | 2020-04-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	wt_AA and pcoumarate_10hr_R1 (F018103).mzid_wt_AA_and_pcoumarate_10hr_R1_(F018103).pride.mgf.gz	Mzid
	wt_AA and pcoumarate_10hr_R1 (F018103).pride.mztab.gz	Mztab
	wt_AA and pcoumarate_10hr_R2 (F018104).mzid_wt_AA_and_pcoumarate_10hr_R2_(F018104).pride.mgf.gz	Mzid
	wt_AA and pcoumarate_10hr_R2 (F018104).pride.mztab.gz	Mztab
	wt_AA and pcoumarate_10hr_R3 (F018105).mzid_wt_AA_and_pcoumarate_10hr_R3_(F018105).pride.mgf.gz	Mzid

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Publications

Response of Pseudomonas putida to Complex, Aromatic-Rich Fractions from Biomass.

Park Mee-Rye MR Chen Yan Y Thompson Mitchell M Benites Veronica T VT Fong Bonnie B Petzold Christopher J CJ Baidoo Edward E K EEK Gladden John M JM Adams Paul D PD Keasling Jay D JD Simmons Blake A BA Singer Steven W SW

ChemSusChem 20200430 17

There is strong interest in the valorization of lignin to produce valuable products; however, its structural complexity has been a conversion bottleneck. Chemical pretreatment liberates lignin-derived soluble fractions that may be upgraded by bioconversion. Cholinium ionic liquid pretreatment of sorghum produced soluble, aromatic-rich fractions that were converted by Pseudomonas putida (P. putida), a promising host for aromatic bioconversion. Growth studies and mutational analysis demonstrated t ...[more]

PMID: 32160408

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