Dataset Information

Egg White as a quality control in MALDI Mass Spectrometry Imaging

ABSTRACT: Tryptic peptides and N-glycans were spatially mapped and visualised on formalin-fixed paraffin-embedded (FFPE) endometrial cancer tissue microarrays (TMAs) using an ultrafleXtreme MALDI-ToF/ToF MS instrument (Bruker Daltonics). FFPE egg white was placed either side of each TMA and used as an external control to monitor detector performance and sample preparation.

INSTRUMENT(S): ultraflex

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Egg

SUBMITTER: Matthew Briggs

LAB HEAD: Peter Hoffmann

PROVIDER: PXD014310 | Pride | 2019-12-11

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20190620_Egg-White-Profiling-Peptides.mgf	Mgf
	20190620_GlycanDigest-OVA.txt	Txt
	20190620_PeptideSummaryReportEggWhiteTrypticPeptidesMALDIMSMS.mht	Other
	GlycanDigestOVADetails.docx	Other
	UFX_UniSA-PMF-00038_LIFT-00119.xml	Xml

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Publications

Egg White as a Quality Control in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI).

Condina Mark R MR Mittal Parul P Briggs Matthew T MT Oehler Martin K MK Klingler-Hoffmann Manuela M Hoffmann Peter P

Analytical chemistry 20191113 23

The strength of MALDI-MSI is to analyze and visualize spatial intensities of molecular features from an intact tissue. The distribution of the intensities can then be visualized within a single tissue section or compared in between sections, acquired consecutively. This method can be reliably used to reveal physiological structures and has the potential to identify molecular details, which correlate with biological outcomes. MALDI-MSI implementation in clinical laboratories requires the ability ...[more]

PMID: 31660720

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