Proteomics

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A protein expression during development of S. coelicolor in liquid medium


ABSTRACT: In this paper we analyzed the S.coelicolor proteome at four points in time as it grew in liquid medium s i using three biological and two technical replicates. The proteome was measured during exponential growth and its entry into the stationary phase at 20, 35, 50 and 65 hours. Differential expression analysis identified key metabolic pathways and their hierarchy of activation during population growth. 4 and 3 pathways were found significantly overrepresented only under 35 to 20 hours or 50 to 35 hours respectively. Six pathways were found to be overrepresented within all three temporal groups (20 to 35 hours, 35 to 50 hours and 50 to 65 hours), with one pathway being significantly overrepresented exclusively in the 50-65 h interval. Central metabolic system enzymes involved in DNA replication were enriched in the late stages while the proteins involved in the transcription and translation machinery were overrepresented in early stages and decreased over time. A strong correlation was found between the expression of about a third of the proteins and mRNA, while another third correlated negatively. The data presented here represents quantitative evidence for the growing populations’ observed behaviour.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Streptomyces Coelicolor

SUBMITTER: Martin Hubalek  

LAB HEAD: Matin Hubalek

PROVIDER: PXD014338 | Pride | 2020-04-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
180522_JP_library12.group Other
180522_JP_library12_MGFPeaklist.mgf Mgf
180522_T20JP1IDA.wiff Wiff
180522_T20JP1IDA.wiff.scan Wiff
180522_T20JP1SW1.wiff Wiff
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Publications

A Comparison of Protein and mRNA Expression during Development of the Soil Dwelling Prokaryote (S. coelicolor).

Pospíšil Jiří J   Strunin Dmytro D   Ziková Alice A   Hubálek Martin M   Vohradský Jiří J  

Proteomics 20200629 14


In this paper, correlation analysis of protein and mRNA levels in the soil dwelling bacteria Streptomyces coelicolor (S. coelicolor M145) is presented during development of the population as it grew in liquid medium using three biological and two technical replicates, measured during exponential growth, and its entry into the stationary phase. The proteome synthesis time series are compared with the gene expression time series measured previously under identical experimental conditions. Results  ...[more]

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