Proteomics

Dataset Information

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QUANTITATIVE SUBCELLULAR PROTEOMICS OF THE ORBITOFRONTAL CORTEX OF SCHIZOPHRENIA PATIENTS

ABSTRACT: We analyzed OFC post-mortem brain tissue from patients classified as residual schizophrenia according to the DMS-IV manual. In this classification, the patients do not manifest prominent positive psychotic symptoms but prevail characteristic disturbance of negative symptoms. Through the comparison of patient´s samples against a pool of control subjects without any history of psychiatric illness we generated an organellar proteome map from different subcellular fractions such as mitochondria (MIT), crude nuclear fraction (NUC) and cytoplasm (CYT) with the aim to monitor the subproteome changes using iTRAQ labelling for relative quantification and Selected Reaction Monitoring (SRM) for targeted quantification.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

DISEASE(S): Schizophrenia

SUBMITTER: Proteomics Unit  

LAB HEAD: Fabio Nogueira

PROVIDER: PXD014350 | Pride | 2019-10-07

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
NIST_human_IT_2012-05-30_7AA.splib Other
SRM_analysis.sky Other
SRM_analysis.sky.view Other
SRM_analysis.skyd Other
TQ_1895_NUC46_1.raw Raw
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Publications

Quantitative Subcellular Proteomics of the Orbitofrontal Cortex of Schizophrenia Patients.

Velásquez Erika E   Martins-de-Souza Daniel D   Velásquez Ingrid I   Carneiro Gabriel Reis Alves GRA   Schmitt Andrea A   Falkai Peter P   Domont Gilberto B GB   Nogueira Fabio C S FCS  

Journal of proteome research 20191028 12

Schizophrenia is a chronic disease characterized by the impairment of mental functions with a marked social dysfunction. A quantitative proteomic approach using iTRAQ labeling and SRM, applied to the characterization of mitochondria (MIT), crude nuclear fraction (NUC), and cytoplasm (CYT), can allow the observation of dynamic changes in cell compartments providing valuable insights concerning schizophrenia physiopathology. Mass spectrometry analyses of the orbitofrontal cortex from 12 schizophre  ...[more]

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