Dataset Information

Melanoma spliced and linear HLA-I peptides

ABSTRACT: In this project we have identifed and linear and spliced HLA-I bound peptides to LM-MEl-44, 53 and 33 cell lines in two different condition, treated and not treated with interferone gamma.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte, Cell Culture

DISEASE(S): Skin Melanoma

SUBMITTER: Pouya Faridi

LAB HEAD: Anthony Purcell

PROVIDER: PXD014397 | Pride | 2020-09-25

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	F120190129_PF_Mel_33_IFN_negative_pool1.mgf	Mgf
	F120190129_PF_Mel_33_IFN_negative_pool1.pride.mgf.gz	Mgf
	F120190129_PF_Mel_33_IFN_negative_pool1.raw	Raw
	F120190129_PF_Mel_33_IFN_negative_pool10.mgf	Mgf
	F120190129_PF_Mel_33_IFN_negative_pool10.pride.mgf.gz	Mgf

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Publications

Spliced Peptides and Cytokine-Driven Changes in the Immunopeptidome of Melanoma.

Faridi Pouya P Woods Katherine K Ostrouska Simone S Deceneux Cyril C Aranha Ritchlynn R Duscharla Divya D Wong Stephen Q SQ Chen Weisan W Ramarathinam Sri H SH Lim Kam Sian Terry C C TCC Croft Nathan P NP Li Chen C Ayala Rochelle R Cebon Jonathan S JS Purcell Anthony W AW Schittenhelm Ralf B RB Behren Andreas A

Cancer immunology research 20200916 10

Antigen recognition by CD8<sup>+</sup> T cells is governed by the pool of peptide antigens presented on the cell surface in the context of HLA class I complexes. Studies have shown not only a high degree of plasticity in the immunopeptidome, but also that a considerable fraction of all presented peptides is generated through proteasome-mediated splicing of noncontiguous regions of proteins to form novel peptide antigens. Here, we used high-resolution mass spectrometry combined with new bioinform ...[more]

PMID: 32938616

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