Proteomics

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Qualitative proteomic comparison of metabolic and CM-like protein fractions in old and modern wheat Italian genotypes by a shotgun approach


ABSTRACT: The close relationship between diet and health is generally recognized and the growing wellness and consciousness, especially in developed countries, have led to increasing interest for old wheat genotypes, based on perceived health benefits. Although nutritional comparison between old and modern wheat varieties is still controversial, it is generally accepted that old wheat genotypes remained unchanged over the last hundred years. By contrast, modern wheat genotypes are derived by modification of old wheats during the so-called “Green-Revolution” in the second half of the 20th century focusing on obtaining properties in terms of higher grain yield. The present work reports the first comprehensive proteomic profiling and qualitative comparison at the molecular level of metabolic and Chloroform-Methanol (CM)-like protein fractions extracted from mature kernels of two old Sicilian durum wheat landraces, Russello and Timilia Reste Bianche, and Simeto, an improved durum wheat variety widespread in Italy and other Mediterranean countries and chosen as representative of the most widely commercial cultivars. The results obtained reveal that metabolic and CM-like protein fractions of old and modern genotypes present remarkably high similarity with only minor differences. This leads to the conclusion that from a food and nutritional perspective there is a substantial equivalence of the protein composition of the old and modern cultivars.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Triticum Aestivum (wheat)

SUBMITTER: Antonella Di Francesco  

LAB HEAD: Salvatore Foti

PROVIDER: PXD014449 | Pride | 2019-10-25

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
DataBase.fasta Fasta
RusselloCML-protein-peptides.xlsx Xlsx
RusselloCML-proteins.xlsx Xlsx
RusselloMP-protein-peptides.xlsx Xlsx
RusselloMP-proteins.xlsx Xlsx
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Publications

Qualitative proteomic comparison of metabolic and CM-like protein fractions in old and modern wheat Italian genotypes by a shotgun approach.

Di Francesco Antonella A   Saletti Rosaria R   Cunsolo Vincenzo V   Svensson Birte B   Muccilli Vera V   Vita Pasquale De P   Foti Salvatore S  

Journal of proteomics 20191016


The close relationship between diet and health is generally recognized and the growing wellness and consciousness, especially in developed countries, have led to increasing interest for old wheat genotypes, based on perceived health benefits. Although nutritional comparison between old and modern wheat varieties is still controversial, it is generally accepted that old wheat genotypes remained unchanged over the last hundred years. By contrast, modern wheat genotypes are derived by modification  ...[more]

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