Dataset Information

The liver proteome in a mouse model for Ascaris suum resistance and susceptibility – evidence for an altered innate immune response

ABSTRACT: Ascariasis is a neglected tropical disease that affects 800 million people worldwide. Whereas most people only experience light worm burden, some people experience heavy worm burdens even after several rounds of chemotherapy, a phenomenon known as predisposition. Such heavy infections are associated with more severe symptoms and increased chronic morbidity. In order to investigate potential mechanisms that may explain the observed predisposition, we infected mice with the porcine ascarid A. suum using an established mouse model with two different mouse strains, C57BL/6J and CBA/Ca, as a model for heavy and light infection. At day 7 post infection we investigated the liver proteome, using shotgun mass spectrometry, of both infected and control mice of each strain. We identified intrinsic differences, between the two mouse strains, in both oxidative phosphorylation proteins and proteins involved in the retinol metabolism. Additionally, we found differences between the two mouse strains in activation of the complement system, where the resistant mouse strain has higher protein abundances for lectin pathway proteins and the susceptible mouse strain has higher protein abundances for complement inhibiting proteins. The resistant mouse strain had a higher abundance of proteins involved in the activation of the complement cascade via the lectin pathway. In contrast, the susceptible strain demonstrated a higher abundance of proteins involved in arresting the complement pathway. In conclusion, we observed clear differences between the two mouse strains both intrinsically and under infection.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: James Carolan

LAB HEAD: James Carolan

PROVIDER: PXD014508 | Pride | 2019-11-12

REPOSITORIES: Pride

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The liver proteome in a mouse model for Ascaris suum resistance and susceptibility: evidence for an altered innate immune response.

Deslyper Gwendoline G Holland Celia V CV Colgan Thomas J TJ Carolan James C JC

Parasites & vectors 20190814 1

<h4>Background</h4>Ascariasis is a neglected tropical disease that affects 800 million people worldwide. Whereas most people only experience light worm burden, some people experience heavy worm burdens even after several rounds of chemotherapy, a phenomenon known as predisposition. Such heavy infections are associated with more severe symptoms and increased chronic morbidity.<h4>Methods</h4>In order to investigate potential mechanisms that may explain the observed predisposition, we infected mic ...[more]

PMID: 31412915

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	C57_7pi_C1.raw	Raw
	C57_7pi_C2.raw	Raw
	C57_7pi_C3.raw	Raw
	C57_7pi_C4.raw	Raw
	C57_7pi_Inf1.raw	Raw