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Metaproteomic data of maize rhizosphere

ABSTRACT: We present metaproteome data from maize rhizosphere from sodic soil. Isolation of proteome from maize rhizosphere collected from Experimental Farm, ICAR-IISS, Mau, India was done with the standardized protocol at our laboratory and metaproteome analysis was done with the standardized pipepline. In total 696 proteins with different functions representing 245 genus and 395 species were identified. The proteome data provides direct evidence on the biological processes in soil ecosystem and is the first reported reference data from maize rhizosphere.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Rhizosphere Soil Bacteria Ensemble

SUBMITTER: Renu S

LAB HEAD: Dr Renu

PROVIDER: PXD014519 | Pride | 2019-10-08

REPOSITORIES: Pride

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	04102016_NBAIM_T2G.raw.zip	Raw
	PEAK.mgf	Mgf
	SEARCH.xlsx	Xlsx

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Publications

Metaproteomic data of maize rhizosphere for deciphering functional diversity.

Renu Gupta Sanjay Kumar SK Rai Ashutosh Kumar AK Sarim Khan Mohd KM Sharma Anu A Budhlakoti Neeraj N Arora Devendra D Verma Dhiraj Kumar DK Singh Dhananjaya P DP

Data in brief 20190927

Metaproteomics is a powerful tool for obtaining data on all proteins recovered directly from environmental samples at a given time. It provides a direct evidence of functional diversity and structure among microbiota present in niches and significant insights into microbial activity together with metabolomics, which is the study of the intermediate and end-products of cellular processes. Metaproteomics is a comparatively new approach which is facing a number of empirical, technical, computationa ...[more]

PMID: 31692741

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Project description:We present metaproteome data from wheat rhizosphere from saline and non-saline soil. For collection and acquisition of metaproteome from wheat rhizosphere under saline and normal conditions, a survey was conducted in regions of Haryana, India. Samples from 65 days old plants (wheat var HD2967) were collected and pooled and based on EC,saline (NBAIM B; EC 6mS cm-1; pH 9.0; Bhaupur 2, Haryana, INDIA; 29°19'8"N;76°48'53"E) and normal soil samples (NBAIM C; EC 200 uS cm-1; pH 7.2; Near Nainform, Haryana, INDIA; 29°19'8"N;76°48'53"E) were selected for isolation of proteome with the standardized protocol at our laboratory followed by metaproteome analysis with the standardized pipepline. In total 1538 and 891 proteins were obtained from wheat rhizosphere from saline and non-saline respectively with the given parameters and software. Among 1410 proteins unique for saline soil, proteins responsible for glycine, serine and threonine metabolism and arginine and proline biosynthesis were found in saline and absent in non-saline. The present study extends knowledge about the physiology and adaptations of the wheat rhizosphere associated microbiota under saline soil.

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