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AP-MS analysis of Secernin-1 (SCRN1)

ABSTRACT: Affinity purification-Mass spectrometry analysis of bioGFP-Secernin-1 in HEK293 cells and in rat brains.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Cell Culture

SUBMITTER: Riccardo Stucchi

LAB HEAD: Casper Hoogenraad

PROVIDER: PXD014534 | Pride | 2019-08-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	AP-MS_SCRN1_HEK293.xlsx	Xlsx
	AP-MS_SCRN1_brain.xlsx	Xlsx
	OR10_20150928_RS_GFP.msf	Msf
	OR10_20150928_RS_GFP_1.raw	Raw
	OR10_20150928_RS_GFP_2.raw	Raw

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1 - 5 of 18

Publications

VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.

Lindhout Feline W FW Cao Yujie Y Kevenaar Josta T JT Bodzęta Anna A Stucchi Riccardo R Boumpoutsari Maria M MM Katrukha Eugene A EA Altelaar Maarten M MacGillavry Harold D HD Hoogenraad Casper C CC

The EMBO journal 20190823 20

In neurons, the continuous and dynamic endoplasmic reticulum (ER) network extends throughout the axon, and its dysfunction causes various axonopathies. However, it remains largely unknown how ER integrity and remodeling modulate presynaptic function in mammalian neurons. Here, we demonstrated that ER membrane receptors VAPA and VAPB are involved in modulating the synaptic vesicle (SV) cycle. VAP interacts with secernin-1 (SCRN1) at the ER membrane via a single FFAT-like motif. Similar to VAP, lo ...[more]

PMID: 31441084

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