Dataset Information

Anaerobic biogas plants via LC-MS/MS

ABSTRACT: Anaerobic degradation (AD) of heterogeneous agricultural substrates is a complex process involving a diverse microbial community. While microbial community composition of a variety of biogas plants (BPs) is well described, little is known about metabolic processes and microbial interaction patterns. Here, we analyzed 16 large-scale BPs using metaproteomics. All metabolic steps of AD were observed in the metaproteome, and multivariate analyses indicated that they were shaped by temperature, pH, volatile fatty acid content and substrate types. Biogas plants can be subdivided into hydrogenotrophic, acetoclastic or a mixture of both methanogenic pathways based on their process parameters, taxonomic and functional metaproteome. Network analyses showed large differences in metabolic and microbial interaction patterns. Both, number of interactions and interaction partners were highly dependent on the prevalent methanogenic pathway for most species. Nevertheless, we observed a highly conserved metabolism of different abundant Pseudomonas spp. for all BPs indicating a key role during AD in carbohydrate hydrolysis irrespectively of variabilities in substrate input and process parameters. Thus, Pseudomonas spp. are of high importance for robust and versatile AD food webs, which highlight a large variety of downstream metabolic processes for their respective methanogenic pathways.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Nico Jehmlich

LAB HEAD: Nico Jehmlich

PROVIDER: PXD014605 | Pride | 2019-11-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170911_NJ_Buettner_BP1_10_5.msf	Msf
	20170911_NJ_Buettner_BP1_10_5.raw	Raw
	20170911_NJ_Buettner_BP1_11_5.msf	Msf
	20170911_NJ_Buettner_BP1_11_5.raw	Raw
	20170911_NJ_Buettner_BP1_12_5.msf	Msf

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Publications

Pseudomonas spp. are key players in agricultural biogas substrate degradation.

Buettner Christian C von Bergen Martin M Jehmlich Nico N Noll Matthias M

Scientific reports 20190906 1

Anaerobic degradation (AD) of heterogeneous agricultural substrates is a complex process involving a diverse microbial community. While microbial community composition of a variety of biogas plants (BPs) is well described, little is known about metabolic processes and microbial interaction patterns. Here, we analyzed 16 large-scale BPs using metaproteomics. All metabolic steps of AD were observed in the metaproteome, and multivariate analyses indicated that they were shaped by temperature, pH, v ...[more]

PMID: 31492882

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