Dataset Information

The liver,spleen,brain,intestine and kidney tissue of the Oreochromis niloticus which infected with Streptococcus agalactiae LC-MSMS

ABSTRACT: The Oreochromis niloticus was infected with S.agalactiae strain YM001, than collected the liver,spleen,brain,intestine and kidney tissue for proteome

INSTRUMENT(S): Q Exactive

ORGANISM(S): Oreochromis Niloticus Niloticus

TISSUE(S): Spleen, Brain, Intestine, Liver, Kidney

SUBMITTER: ming chen

LAB HEAD: Fish disease control and prevention laboratory

PROVIDER: PXD014616 | Pride | 2020-05-26

REPOSITORIES: Pride

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Publications

The Interaction between Phagocytes and Streptococcus agalactiae (GBS) Mediated by the Activated Complement System is the Key to GBS Inducing Acute Bacterial Meningitis of Tilapia.

Liu Yu Y Li Liping L Huang Ting T Wu Wende W Liang Wanwen W Chen Ming M

Animals : an open access journal from MDPI 20191016 10

Streptococcus agalactiae is an important pathogen for tilapia meningitis. Most of the infected tilapia die rapidly in production, when the way to study the pathogenic mechanism of bacteria on host through chronic infection in laboratory is not comprehensive and accurate enough to elucidate the real pathogenic mechanism. The objective of this study was to investigate the mechanism of acute bacterial meningitis of tilapia caused by Streptococcus agalactiae (GBS), and provide a theore ...[more]

PMID: 31623233

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Project description:ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the role of ClpP on Enterococcus faecalis is still obscure. A clpP deletion mutant (△clpP) was constructed in E. faecalis strain OG1RF in order to elucidate a more comprehensive picture of the effect of ClpP on E. faecalis. Then the global RNA level of genes was detected by RNA sequencing and the global abundance of proteins was determined by mass spectrometer with Tandem Mass Tags (TMT) labeling. We found the ΔclpP mutant strain showed impaired growth at 20℃ or 45℃, at 5% NaCl or 2 mM H2O2. The surviving bacteria of the ΔclpP mutant strain was decreased after exposure to the high concentration of linezolid or minocycline (100 x MIC) for 96 h compared with that of the parent strain. The ΔclpP mutant strain also demonstrated decreased biofilm formation, but increased virulence in a Galleria mellonella model. The abundances of heat shock proteins, regulatory protein Spx (spxA), heat-inducible transcription repressor HrcA, transcriptional regulator CtsR, ATPase/chaperone ClpC and chaperonin GroEL increased in the ΔclpP mutant strain. The FsrA response regulator and its regulating protein gelatinase GelE, acetyl esterase/lipase, adapter protein MecA and fructose-1,6-bisphosphatase (fbp) also up regulated in the ΔclpP mutant strain. However, the abundances of ribosomal protein L4/L1 family protein (rplD) , ribosomal protein L7/L12 (rplL2), 50S ribosomal protein L13 (rplM), L18 (rplR), L20 (rplT), 30S ribosomal protein S14 (rpsN2) and S18 (rpsR) all reduced in the ΔclpP mutant strain. The dihydroorotase (pyrC), orotate phosphoribosyltransferase (pyrE) and orotidine-5'-phosphate decarboxylase (pyrF) also down regulated in the ΔclpP mutant strain. In conclusion, these results suggest that ClpP participates in stress tolerance, biofilm formation, antimicrobials tolerance, and virulence of E. faecalis.

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Dataset Information

The liver,spleen,brain,intestine and kidney tissue of the Oreochromis niloticus which infected with Streptococcus agalactiae LC-MSMS

Dataset's files

Publications

The Interaction between Phagocytes and <i>Streptococcus agalactiae</i> (GBS) Mediated by the Activated Complement System is the Key to GBS Inducing Acute Bacterial Meningitis of Tilapia.

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