Dataset Information

A peptidomimetic proprotein convertase inhibitor is a potential anti-glioma drug as revealed by biological assays and proteomic studies

ABSTRACT: Proprotein convertases (PCs) are proteases which have an important regulatory function in a wide variety of biological processes. However, these enzymes have a key role in the activation of many cancer-related proteins and promotes the malignant phenotype of cancer cells. Here, with proteomics and biological tests, we investigated the consequences of the use of a peptidomimetic PCs inhibitor on glioma cells and macrophages in the same time and its potential application to cancer therapy. Thus, we demonstrated that the PCs inhibitor acts on macrophage activation, characterized by the secretion of pro-inflammatory and anti-tumoral factor. Also, the PCs inhibitor triggers a strong decrease of the proliferation and invasion of glioma cells, even when they are associated with macrophages in spheroids. In fact, the PCs inhibitor induces great changes in the intracellular and secreted protein profiles of the glioma cells. Many of cancerous processes are affected, such as cell growth, proliferation, angiogenesis and the immunosuppressive capacity of cancer cells. Taken together, these data, establish that the inhibitor acts both on cancer cells and macrophages and can be used as a potential anti-cancer therapeutic strategy.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Glioblastoma

SUBMITTER: Soulaimane Aboulouard

LAB HEAD: Michel Salzet

PROVIDER: PXD014679 | Pride | 2020-05-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MSMS-file-C6secretome.zip	Other
	MSMS-file-WTcells.zip	Other
	MSMS-file-WTsecretome.zip	Other
	MSMS-files-C6cells.zip	Other
	RawData-C6cells.zip	Other

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Publications

The Role of a Proprotein Convertase Inhibitor in Reactivation of Tumor-Associated Macrophages and Inhibition of Glioma Growth.

Rose Mélanie M Duhamel Marie M Aboulouard Soulaimane S Kobeissy Firas F Le Rhun Emilie E Desmons Annie A Tierny Dominique D Fournier Isabelle I Rodet Franck F Salzet Michel M

Molecular therapy oncolytics 20200331

Tumors are characterized by the presence of malignant and non-malignant cells, such as immune cells including macrophages, which are preponderant. Macrophages impact the efficacy of chemotherapy and may lead to drug resistance. In this context and based on our previous work, we investigated the ability to reactivate macrophages by using a proprotein convertases inhibitor. Proprotein convertases process immature proteins into functional proteins, with several of them having a role in immune cell ...[more]

PMID: 32300641

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