Dataset Information

Effects of simulated space radiations on the tomato root proteome

ABSTRACT: Plant cultivation on spacecraft or planetary outposts is a promising and actual perspective both for food and bioactive molecules production. To this aim, plants response to ionizing radiations, as an important component of space radiation, must be assessed through on-ground experiments due to the potentially fatal effects on living systems. Hereby we investigated the effects of X-rays and -rays exposure on tomato ‘hairy root’ cultures (HRCs), which represent a solid platform for the production of pharmaceutically relevant molecules, including metabolites and recombinant proteins. In a space application perspective, we used a HRC system previously fortified through the accumulation of anthocyanins, which are known for their anti-oxidant properties. Roots were independently exposed to different photon radiations, namely X-rays (250 kV) and -rays (Co60, 1.25 MeV), both at the intensity of 0.5, 5 and 10 Gy. Molecular changes induced in the proteome of HRCs were investigated by a comparative approach based on Two-Dimensional Difference In-Gel electrophoresis (2D-DIGE) technology and mass spectrometry.

INSTRUMENT(S): LTQ, Q Exactive

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Hairy Root Culture

SUBMITTER: anna maria salzano

LAB HEAD: Andrea Scaloni

PROVIDER: PXD014748 | Pride | 2019-09-25

REPOSITORIES: Pride

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Effects of Simulated Space Radiations on the Tomato Root Proteome.

Desiderio Angiola A Salzano Anna Maria AM Scaloni Andrea A Massa Silvia S Pimpinella Maria M De Coste Vanessa V Pioli Claudio C Nardi Luca L Benvenuto Eugenio E Villani Maria Elena ME

Frontiers in plant science 20191024

Plant cultivation on spacecraft or planetary outposts is a promising and actual perspective both for food and bioactive molecules production. To this aim, plant response to ionizing radiations, as an important component of space radiation, must be assessed through on-ground experiments due to the potentially fatal effects on living systems. Hereby, we investigated the effects of X-rays and γ-rays exposure on tomato "hairy root" cultures (HRCs), which represent a solid platform for the production ...[more]

PMID: 31708949

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