Dataset Information

Charaterising the surfaceome of primary equine chondrocytes

ABSTRACT: The aim of this study was to characterize the surfaceome of primary equine chondrocytes isolated from healthy joints following exposure to the pro-inflammatory cytokines interleukin-1-beta (IL-1β) and tumour necrosis factor-alpha (TNF-α). We employed combined methodology that we recently developed for investigating the surfaceome in stem cells. Membrane proteins were isolated using an aminooxy-biotinylation technique and analysed by mass spectrometry using high throughput shotgun proteomics. Amongst the 431 unique cell surface proteins identified, a high percentage of low-abundance proteins, such as ion channels, receptors and transporter molecules were detected. A high number of proteins exhibited different levels of expression following chondrocyte stimulation with pro-inflammatory cytokines. LPR-1, thrombospondin, VDAC1-2 and annexin A1 were chosen for further analysis and validation by western blotting as proteins of special interest. Our results provide, for the first time, a repository for proteomic data on differentially expressed low-abundance membrane proteins on the surface of chondrocytes in response to pro-inflammatory stimuli.

INSTRUMENT(S): amaZon ETD

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Chondrocyte, Articular Cartilage

DISEASE(S): Osteoarthritis

SUBMITTER: David Boocock

LAB HEAD: David Boocock

PROVIDER: PXD014773 | Pride | 2020-07-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Bjeql_control 1ul_RA3_01_2740peptides_1_1_0.pride.mztab.gz	Mztab
	Bjeql_control 2ul_RA3_01_2741peptides_1_1_0.pride.mztab.gz	Mztab
	Bjeql_control%201ul_RA3_01_2740.pride.mgf.gz	Mgf
	Bjeql_control%202ul_RA3_01_2741.pride.mgf.gz	Mgf
	Bjeql_control1ul_RA3_01_2740.mzML	Mzml

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Publications

Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines.

Jeremiasse Bernadette B Matta Csaba C Fellows Christopher R CR Boocock David J DJ Smith Julia R JR Liddell Susan S Lafeber Floris F van Spil Willem E WE Mobasheri Ali A

BMC molecular and cell biology 20200626 1

<h4>Background</h4>Chondrocytes are exposed to an inflammatory micro-environment in the extracellular matrix (ECM) of articular cartilage in joint diseases such as osteoarthritis (OA) and rheumatoid arthritis (RA). In OA, degenerative changes and low-grade inflammation within the joint transform the behaviour and metabolism of chondrocytes, disturb the balance between ECM synthesis and degradation, and alter the osmolality and ionic composition of the micro-environment. We hypothesize that chond ...[more]

PMID: 32586320

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