Dataset Information

DETECTION OF ODF1 IN KIDNEY MEDULLA

ABSTRACT: ODF1 has been described as an exclusively expressed testicular protein and is located in the outer dense fibers along the sperm tail. ODF1 has been involved in the sperm motility and in the development of the flagellum, but the function of ODF1 is not already clear. Applying western blot, immunofluorescence, and RT-PCR techniques we identified the protein and mRNA of ODF1 in the rat kidney. Finally, the presence of ODF1 in kidney has been confirmed through proteomic analysis using mass spectrometry. The results derived from these different complementary approaches indicate that, to our knowledge and for the first time, ODF1 is demonstrated to be present in an additional organ different to testis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Testis, Kidney

SUBMITTER: María Eugenia Cabrillana

LAB HEAD: Miguel Walter Fornés

PROVIDER: PXD014848 | Pride | 2020-05-27

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	KidneyMedullaCytoskeletonFraction.pdResult	Other
	KidneyMedullaCytoskeletonFraction.raw	Raw
	KidneyMedullaNon-CytoskeletonFraction.raw	Raw
	KidneyMedullaNon-Cytoskeletonfraction.pdResult	Other
	Testis.pdResult	Other

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Publications

ODF1, sperm flagelar protein is expressed in kidney collecting ducts of rats.

Cabrillana M E ME Bocanegra V V Monclus M A MA Lancellotti Te Saez TS Simón L L Funes A K AK Colombo R R Ruiz Estrabón M M Vincenti A E AE Oliva R R Fornés M W MW

Heliyon 20191204 12

ODF1 has been described as an exclusively expressed testicular protein and is located in the outer dense fibers along the sperm tail. ODF1 has been involved in the sperm motility and in the development of the flagellum, but the function of ODF1 is not already clear. Other ODF proteins, such as ODF2 have been characterized in other tissues like the basal body of the kidney primary cilium, but so far only the mRNA of ODF1 has been described in other tissues. These observations let us to hypothesiz ...[more]

PMID: 31867458

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