Proteomics

Dataset Information

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Protein compositions of extracellular vesicles released from Staphylococcus aurues JE2 and its lgt mutant


ABSTRACT: This project detected the protein composition of extracellular vesicles (EV) produced by S. aureus WT JE2 strain and its lgt mutant (lipoprotein deficient mutant). Our recent study found that mutation of lgt in S. aureus USA300 JE2 strain significantly increased EV production in S. aureus and decreased the pore-forming toxin content of vesicles, indicatiung that lipoproteins modulate the EV biogenesis of S. aureus. To gain a better understanding of this process, we detected the protein compositions associated with EVs purified from both WT and lgt mutant EVs by LC-MS/MS. Compared to WT EVs, more cytosoli proteins and less extracellular proteins were identified in lgt mutant EVs. A total of 180 and 198 proteins were identified from WT and lgt mutant EVs, respectively.In addition 27.2% and 33.8% of identified proteins were unique to WT or lgt mutant EVs respectively.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Xiaogang Wang  

LAB HEAD: Jean C. Lee

PROVIDER: PXD014888 | Pride | 2021-09-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
IdentificationresultofJE2EVs1.raw Raw
IdentificationresultoflgtMutantEVs1.raw Raw
JE2EVs2.raw Raw
identificationpeptideresultofWTEVs1.csv Csv
identificationpeptideresultoflgtmutantEVs1.csv Csv
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Publications

<i>Staphylococcus aureus</i> Extracellular Vesicles: A Story of Toxicity and the Stress of 2020.

Wang Xiaogang X   Koffi Paul F PF   English Olivia F OF   Lee Jean C JC  

Toxins 20210120 2


<i>Staphylococcus aureus</i> generates and releases extracellular vesicles (EVs) that package cytosolic, cell-wall associated, and membrane proteins, as well as glycopolymers and exoproteins, including alpha hemolysin, leukocidins, phenol-soluble modulins, superantigens, and enzymes. <i>S. aureus</i> EVs, but not EVs from pore-forming toxin-deficient strains, were cytolytic for a variety of mammalian cell types, but EV internalization was not essential for cytotoxicity. Because <i>S. aureus</i>  ...[more]

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