Proteomics

Dataset Information

0

An Integrated Transcriptome and Proteome Analysis Reveals New Insights of Fruit Russeting Formation in ‘Golden Delicious’ Apple


ABSTRACT: Russeting of apple fruit is a non-invasive physiological disorder. It occurs mainly in 'Golden Delicious' apple and its hybrids, while understanding of its molecular mechanism is still limited. In this study, we used mRNA sequencing and an isobaric tag for relative and absolute quantitation-based quantitative (iTRAQ) proteomic analysis to detect changes in the expression levels of genes and proteins during russeting formation in russeted and non-russeted skin of 'Golden Delicious' apple. We set up three comparison groups representing the three developmental stages in the russeting formation process. With the formation of fruit russeting, there were 2856 differentially expressed genes and 942 differentially expressed proteins in the comparison groups as detected at the transcript level and protein level, respectively. A correlation analysis of the transcriptome and proteome data revealed related-genes involved in lignin biosynthesis are significant changes at different developmental stages during apple russeting formation. Some other transcription factors, such as MYBs, NACs and LIMs were also involved in apple russeting formation. In this study, one LIM transcription factor was preliminarily determined to be involved in lignin biosynthesis by combining to PAL-box element. Studying the identified genes and proteins will provide further insights into the molecular mechanisms controlling apple russeting formation.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Rosaceae

TISSUE(S): Fruit Body

SUBMITTER: Gao-peng Yuan  

LAB HEAD: Pei-hua Cong

PROVIDER: PXD014960 | Pride | 2019-09-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F18FTSNCKF2851_rep1.dat Other
F18FTSNCKF2851_rep2.dat Other
F18FTSNCKF2851_rep3.dat Other
P180632_1_1.raw Raw
P180632_1_10.raw Raw
Items per page:
1 - 5 of 63
altmetric image

Publications

An Integrated Transcriptome and Proteome Analysis Reveals New Insights into Russeting of Bagging and Non-Bagging "Golden Delicious" Apple.

Yuan Gaopeng G   Bian Shuxun S   Han Xiaolei X   He Shanshan S   Liu Kai K   Zhang Caixia C   Cong Peihua P  

International journal of molecular sciences 20190910 18


Apple skin russeting naturally occurs in many varieties, particularly in "Golden Delicious" and its pedigree, and is regarded as a non-invasive physiological disorder partly caused by excessive deposition of lignin. However, the understanding of its molecular mechanism is still limited. In this study, we used iTRAQ (isobaric tags for relative and absolute quantitation) and RNA-seq to detect the changes in the expression levels of genes and proteins in three developmental stages of russeting form  ...[more]

Similar Datasets

2016-10-13 | PXD004568 | Pride
2009-06-16 | E-TABM-514 | biostudies-arrayexpress
2010-11-05 | E-GEOD-24523 | biostudies-arrayexpress
2024-03-06 | E-MTAB-13465 | biostudies-arrayexpress
2015-06-25 | E-GEOD-59947 | biostudies-arrayexpress
2019-11-04 | GSE136842 | GEO
2018-12-25 | GSE115664 | GEO
2019-06-18 | PXD011132 | Pride
2022-04-04 | PXD018885 | Pride
2016-01-18 | E-MTAB-4110 | biostudies-arrayexpress