Dataset Information

Insights into Early Recovery from Influenza Pneumonia by Spatial and Temporal Quantification of Putative Lung Regeneration Cells and buy Lung Proteomics

ABSTRACT: During influenza pneumonia, the alveolar epithelial cells of the lungs are targeted by influenza virus. The distal airway stem cells (DASCs) and proliferating alveolar type II (AT2) cells are reported to be putative lung repair cells. However, their relative spatial and temporal distribution is still unknown during influenza-induced acute lung injury. Here, we investigated the distribution of these cells, and concurrently performed global proteomic analysis of the infected lungs to elucidate and link the cellular and molecular events during influenza pneumonia recovery. BALB/c mice were infected with a sub-lethal dose of influenza H1N1 virus. From 5 to 25 days post-infection (dpi), mouse lungs were subjected to histopathologic and immunofluorescence analysis to probe for global distribution of lung repair cells (using P63 and KRT5 markers for DASCs; PCNA and SPC markers for AT2 cells). At 7 and 15 dpi, infected mouse lungs were also subjected to protein mass spectrometry for relative protein quantification. DASCs appeared only in the damaged area of the lung from 7 dpi onwards, reaching a peak at 21 dpi, and persisted at 25 dpi. However, no differentiation of DASCs to AT2 cells was observed by 25 dpi. In contrast, AT2 cells began proliferating from 7 dpi to replenish its population. Mass spectrometry and gene ontology analysis revealed prominent innate immune response at 7 dpi, which shifted towards adaptive immune responses by 15 dpi. Hence, proliferating AT2 cells but not DASCs contribute to AT2 cell regeneration following transition from innate to adaptive immune responses during the early phase of recovery from influenza pneumonia up to 25 dpi.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Lung, Epithelial Cell

DISEASE(S): Influenza

SUBMITTER: Joe Ong

LAB HEAD: Vincent T. K. Chow

PROVIDER: PXD014967 | Pride | 2020-04-17

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	QPG_F_P5_151113_01_IS367_IEF1.raw	Raw
	QPG_F_P5_151113_01_IS367_IEF12fractions.fasta	Fasta
	QPG_F_P5_151113_01_IS367_IEF12fractions.msf	Msf
	QPG_F_P5_151113_01_IS367_IEF12fractions.mzML	Mzml
	QPG_F_P5_151113_01_IS367_IEF12fractions.mzid.gz	Mzid

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Publications

Insights into Early Recovery from Influenza Pneumonia by Spatial and Temporal Quantification of Putative Lung Regenerating Cells and by Lung Proteomics.

Ong Joe Wee Jian JWJ Tan Kai Sen KS Ler Siok Ghee SG Gunaratne Jayantha J Choi Hyungwon H Seet Ju Ee JE Chow Vincent T VT

Cells 20190826 9

During influenza pneumonia, the alveolar epithelial cells of the lungs are targeted by the influenza virus. The distal airway stem cells (DASCs) and proliferating alveolar type II (AT2) cells are reported to be putative lung repair cells. However, their relative spatial and temporal distribution is still unknown during influenza-induced acute lung injury. Here, we investigated the distribution of these cells, and concurrently performed global proteomic analysis of the infected lungs to elucidate ...[more]

PMID: 31455003

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