Proteomics

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Indole-3-acetic acid degradation in a novel strain Pseudomonas composti LY1


ABSTRACT: Indole-3-acetic acid (IAA), knows as common plant hormone, is one of the most distributed indole derivatives in the environment. A novel strain, which was able to use IAA as sole source of carbon and nitrogen, was isolated from farm soil, identified and classified as Pseudomonas composti LY1 based on 16S rRNA sequence and genome analysis. The optimal growth conditions for LY1 with IAA are characterized. Proteome profile of strain LY1 to IAA and citrate were analyzed and compared using label free strategy with LC-MS/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Pseudomonas Composti

TISSUE(S): Cell Culture

SUBMITTER: Hao Yu  

LAB HEAD: Hao Yu

PROVIDER: PXD014968 | Pride | 2021-09-08

REPOSITORIES: Pride

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Publications

Label-Free Quantitative Proteomic Analysis of the Global Response to Indole-3-Acetic Acid in Newly Isolated <i>Pseudomonas</i> sp. Strain LY1.

Zhao Shuxue S   Chen Xi X   Sun Qianshu Q   Wang Fei F   Hu Chunhui C   Guo Lizhong L   Bai Jie J   Yu Hao H  

Frontiers in microbiology 20210810


Indole-3-acetic acid (IAA), known as a common plant hormone, is one of the most distributed indole derivatives in the environment, but the degradation mechanism and cellular response network to IAA degradation are still not very clear. The objective of this study was to elucidate the molecular mechanisms of IAA degradation at the protein level by a newly isolated strain <i>Pseudomonas</i> sp. LY1. Label-free quantitative proteomic analysis of strain LY1 cultivated with IAA or citrate/NH<sub>4</s  ...[more]

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