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An efficient extraction of intervertebral disc (IVD) proteins coupled with the Liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection

ABSTRACT: A specific discovery proteomic protocol for intervertebral discs relies on an efficient protein extraction using the bead beating homogenizing method with stainless steel grinding balls coupled with a customized lysis buffer, and followed by the protein detection with specific mass spectrometry setting.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Intervertebral Disc

SUBMITTER: Chuen Lam

LAB HEAD: Thomas Chuen Lam

PROVIDER: PXD015033 | Pride | 2019-09-11

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20170923_CalMix_SC_5ul_1.9ug_IDA1.wiff	Wiff
	20170923_CalMix_SC_5ul_1.9ug_IDA1.wiff.scan	Wiff
	20170923_CalMix_SC_5ul_1.9ug_IDA1_2_3combined.group	Other
	20170923_CalMix_SC_5ul_1.9ug_IDA1_2_3combined.mgf	Mgf
	20170923_CalMix_SC_5ul_1.9ug_IDA2.wiff	Wiff

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