Dataset Information

HnRNP M and HSP 90α as Potential Biomarkers in Early Stage Cutaneous Head and Neck Melanoma Tissue

ABSTRACT: Background: Timely diagnosis is important for successful treatment of cutaneous melanoma. Currently, Breslow tumor thickness and mitotic rate are used for malignant melanoma classification and prognosis, but these parameters can assess disease progression risk only to a certain degree. Therefore, there is a need for new melanoma protein biomarkers that would aid early and accurate diagnosis and prediction of their metastatic potential. Methodology and Findings: This retrospective case control study is based on proteomic profiling of formalin-fixed archival tissues of 31 early-stage head and neck cutaneous malignant melanoma samples using liquid chromatography / mass spectrometry. A melanoma proteomic profile was identified and protein expression levels were compared to the proteome profile of melanocytic naevi and correlated to established prognostic factors and disease-specific survival. In accordance with the American Joint Committee on Cancer guidelines, recursive partitioning multivariate analysis was used to identify potential biomarkers associated with metastatic potential of early-melanoma. Heterogeneous nuclear ribonucleoprotein M and heat shock protein 90 alpha were profiled as independent prognostic factors. Their elevated expression was clinically relevant for predicting an exceedingly high metastatic hazard ratio. These proteins were superior in estimating disease progression risk when compared to Breslow thickness and mitotic rate. Conclusions and Significance: Identification of biomarkers in early stage cutaneous head and neck melanoma is an important step towards predicting metastatic potential and prognosis of the disease. Clinical confirmation and further validation of the proteins identified in this study would provide a novel tool for identifying patients at risk for developing metastatic disease.

INSTRUMENT(S): LTQ Orbitap Discovery

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Skin

DISEASE(S): Head And Neck Cancer

SUBMITTER: Ruđer Novak

LAB HEAD: Lovorka Grgurević

PROVIDER: PXD015137 | Pride | 2019-11-27

REPOSITORIES: Pride

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Tumor tissue hnRNP M and HSP 90α as potential predictors of disease-specific mortality in patients with early-stage cutaneous head and neck melanoma: A proteomics-based study.

Košec Andro A Novak Ruđer R Konjevoda Paško P Trkulja Vladimir V Bedeković Vladimir V Grgurević Lovorka L

Oncotarget 20191119 62

<h4>Background</h4>Breslow tumor thickness and mitotic rate are standardly used for risk stratification of patients with malignant melanoma. However, their prognostic value is relatively limited and a need for improved prognostication has been advocated. We aimed to screen the tumor tissue proteome in a search for potentially useful prognostic factors in early-stage cutaneous head and neck melanoma.<h4>Methodology and findings</h4>Proteomic profiles of archival formalin-fixed tissue samples of 3 ...[more]

PMID: 31803364

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