Dataset Information

Label-free quantitative proteomics versus antibody-based assays to measure neutrophil derived enzymes in saliva

ABSTRACT: Discovery-based quantitative proteomics identified dozens of novel protein candidates in saliva, yet none are clinically qualified. Mass spectrometry (MS) has been recognized as newer 'gold standard' for the identification and analysis of individual proteins in complex biological samples due to higher sensitivity, specificity and independence of antibody availability. However, whether MS can replace immunoassays for salivary biomarker validation has not been thoroughly studied. Therefore, we aimed to test the potential of label-free quantitative (LFQ) proteomics in comparison to conventional antibody-based methods to quantify ‘candidate biomarkers’ in saliva during experimentally-induced oral inflammation development in humans. Whole unstimulated saliva, collected at five time points, was analyzed by LFQ shotgun proteomics. Salivary levels of ‘candidate biomarkers’ were also assessed by antibody-based immunoassays such as ELISA and time-resolved immunofluorometric assay (IFMA).

INSTRUMENT(S): LCQ Classic, Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Saliva

DISEASE(S): Gingivitis

SUBMITTER: Kai Bao

LAB HEAD: Nagihan Bostanci

PROVIDER: PXD015220 | Pride | 2019-12-20

REPOSITORIES: pride

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Label-Free Quantitative Proteomics versus Antibody-Based Assays to Measure Neutrophil-Derived Enzymes in Saliva.

Silbereisen Angelika A Alassiri Saeed S Bao Kai K Grossmann Jonas J Nanni Paolo P Fernandez Claudia C Tervahartiala Taina T Nascimento Gustavo G GG Belibasakis Georgios N GN Heikkinen Anna-Maria AM Lopez Rodrigo R Sorsa Timo T Bostanci Nagihan N

Proteomics. Clinical applications 20200107 3

<h4>Purpose</h4>This study aims to validate label-free quantitative proteomics (LFQ) against antibody-based methods for quantifying established periodontal disease biomarkers in saliva.<h4>Experimental design</h4>In an experimental gingivitis model, healthy volunteers (n = 10) provide saliva at baseline (d0), during the induction (d7, d14, d21) and resolution (d35) of gingival inflammation (total n = 50). Biomarker levels are analyzed by LFQ and time-resolved immunofluorometric assay (IFMA) or e ...[more]

PMID: 31840410

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