Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive Plus
ORGANISM(S): Bos Taurus (bovine)
SUBMITTER: henrik molina
LAB HEAD: Pena, John T.G.
PROVIDER: PXD015234 | Pride | 2019-11-18
REPOSITORIES: Pride
Items per page: 5 1 - 5 of 5 |
Gupta Mrinali P MP Tandalam Sangeetha S Ostrager Shariss S Lever Alexander S AS Fung Angus R AR Hurley David D DD Alegre Gemstonn B GB Espinal Jasmin E JE Remmel H Lawrence HL Mukherjee Sushmita S Levine Benjamin M BM Robins Russell P RP Molina Henrik H Dill Brian D BD Kenific Candia M CM Tuschl Thomas T Lyden David D D'Amico Donald J DJ Pena John T G JTG
Nature methods 20191111 12
Extracellular vesicles (EVs) are secreted nanosized particles with many biological functions and pathological associations. The inability to image EVs in fixed tissues has been a major limitation to understanding their role in healthy and diseased tissue microenvironments. Here, we show that crosslinking mammalian tissues with formaldehyde results in significant EV loss, which can be prevented by additional fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) for visualization of E ...[more]