Proteomics

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Comparative TMT-based quantitative proteomic analysis of the Tachaea chinensis isopod during parasitism


ABSTRACT: Parasitic isopods perforate and attach to the host integument via the mandibles, then feed on hemolymph and exudate from the wounds. Such isopods attack a variety of commercially important fish and crustacean hosts. Like other hematophagous parasites, isopods employ biomolecules that inhibit host blood conglutination and defense. In the present study, a tandem mass tag-based quantitative proteomic approach was used to identify differentially expressed proteins in Tachaea chinensis parasites of shrimp, by comparing parasitic (fed) and pre-parasitic (unfed) individuals. We identified 888 proteins from 1510 total peptides, with a significant difference in 129 between the fed and unfed groups. Among these, 37 were upregulated and 92 were downregulated in unfed T. chinensis. This indicates that T. chinensis may require more energy before parasitism during its search for a host. In addition, as is the case for other blood-sucking parasites, it might secrete antihemostatic, anti-inflammatory and immunomodulatory molecules to facilitate blood meal acquisition. Our study is the first to use a TMT-based proteomic approach to analyze the proteome of isopod parasites, and our results will facilitate our understanding of the molecular mechanisms of isopod parasitism on crustaceans.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Tachaea Chinensis

TISSUE(S): Whole Body

SUBMITTER: Yingdong Li  

LAB HEAD: Yingdong Li

PROVIDER: PXD015247 | Pride | 2020-05-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
PSN19003-4.msf Msf
PSN19003-F1.raw Raw
PSN19003-F10.raw Raw
PSN19003-F2.raw Raw
PSN19003-F3.raw Raw
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Publications

Comparative Tandem Mass Tag-Based Quantitative Proteomic Analysis of <i>Tachaea chinensis</i> Isopod During Parasitism.

Li Yingdong Y   Li Xin X   Han Zhibin Z   Xu Weibin W   Li Xiaodong X   Chen Qijun Q  

Frontiers in cellular and infection microbiology 20191011


Parasitic isopods perforate and attach to the host integument via the mandibles and then feed on hemolymph and exudate from the wounds. Such isopods attack a variety of commercially important fish and crustacean hosts. Similar to other hematophagous parasites, isopods may also employ biomolecules that affect host blood conglutination and defense systems. In the present study, a tandem mass tag-based quantitative proteomic approach was used to identify differentially expressed proteins in <i>Tach  ...[more]

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