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Humoral Immune response of Galleria mellonella to BCG and APP

ABSTRACT: The humoral immune response of Galleria mellonella exposed to Mycobacterium bovis BCG (vaccine strain), 1x107 CFU (10 ul inoculum) were injected into the larvae. For low dose BCG challenge, 1x105 CFU (10 ul) were injected. For larval challenge with Actinobacillus pleuropneumoniae (APP), ~1.5x106 CFU (10 ul) were injected. APP were only challenged at this single dose. For larval challenge with Control (0h) were included. APP and low dose (1x105 CFU) BCG were sampled only at 48 h post infection.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Galleria Mellonella

SUBMITTER: Gerard Sheehan

LAB HEAD: Prof Kevin Kavanagh

PROVIDER: PXD015250 | Pride | 2021-09-08

REPOSITORIES: Pride

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Innate Immune Responses of Galleria mellonella to Mycobacterium bovis BCG Challenge Identified Using Proteomic and Molecular Approaches.

Asai Masanori M Sheehan Gerard G Li Yanwen Y Robertson Brian D BD Kavanagh Kevin K Langford Paul R PR Newton Sandra M SM

Frontiers in cellular and infection microbiology 20210209

The larvae of the insect Galleria mellonella, have recently been established as a non-mammalian infection model for the Mycobacterium tuberculosis complex (MTBC). To gain further insight into the potential of this model, we applied proteomic (label-free quantification) and transcriptomic (gene expression) approaches to characterise the innate immune response of G. mellonella to infection with Mycobacterium bovis BCG lux over a 168 h time course. Proteomic analy ...[more]

PMID: 33634038

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