Proteomics

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Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology


ABSTRACT: Transplantation is currently the best treatment option for end-stage renal disease (ESRD) patients. However, acute rejection (AR) is the main source of failure in renal transplantation. The current gold standard for diagnosis of AR involves renal biopsy, but it is invasive, time consuming, costly and inconvenient. Sensitive and less invasive detection of AR episodes in renal transplant patients is essential to preserve the allograft function. Here, we applied isobaric tags for relative and absolute quantisation (iTRAQ) mass spectrometry to analyse serum protein expression in AR patients and healthy controls. Overall, 1399 proteins were identified. Using a cut-off of Q<0.05 and a fold change > 1.2 for the expressed variation, 109 proteins showed distinct differential expression between the AR and control groups, of whom 72 were upregulated and 37 were downregulated. Several proteins, such as properdin, keratin 1, lipoprotein (a) and vitamin D-binding protein, may play roles in the pathogenesis of AR. This study focused on iTRAQ-based proteomic profiling of serum samples in AR. Insights from our work might help advance our understanding of the molecular mechanisms of AR and identify potential novel biomarkers of AR for further characterization.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Yue Zhang  

LAB HEAD: Yong Dai

PROVIDER: PXD015336 | Pride | 2021-09-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F15FTSSCKF2968_I-cIzOj007_rep1.dat Other
P150573_1.wiff Wiff
P150573_1.wiff.scan Wiff
P150573_10.wiff Wiff
P150573_10.wiff.scan Wiff
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Publications

Proteomic analysis of differentially expressed proteins in the serum of patients with acute renal allograft rejection using iTRAQ labelling technology.

Zhang Yue Y   Ou Minglin M   Lin Hua H   Lai Liusheng L   Chen Huaizhou H   Chen Jiejing J   Sui Weiguo W   Xue Wen W   Zhang Ruohan R   Gan Qing Q   Tang Donge D   Sun Xuyong X   Dong Jianhui J   Yan Qiang Q   Dai Yong Y  

Molecular medicine reports 20200706 3


Transplantation is currently the best treatment for patients with end‑stage renal disease. However, acute rejection (AR) is the major source of failure in renal transplantation. The current best practice for the diagnosis of AR involves renal biopsy, but it is invasive, time‑consuming, costly and inconvenient. Sensitive and less invasive detection of AR episodes in renal transplant patients is essential to preserve allograft function. The present study applied isobaric tags for relative and abso  ...[more]

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