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Global proteomic profiling of primary macrophages during M. tuberculosis infection identifies TAX1BP1 as a mediator of autophagy targeting

ABSTRACT: We profiled changes in the macrophage proteome during a time-course of primary murine bone marrow-derived macrophages infected with Mycobacterium tuberculosis. We measured changes in global protein abundance, phosphorylation, and ubiquitylation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bone Marrow-derived Macrophage, Macrophage

DISEASE(S): Tuberculosis

SUBMITTER: Jonathan Budzik

LAB HEAD: Jeffery Scott Cox

PROVIDER: PXD015361 | Pride | 2020-02-17

REPOSITORIES: Pride

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			Action	DRS
	20170515-jb-35-58-PH-evidence-David-database.txt	Txt
	20170515-jb-35-58-evidence-search-1.txt	Txt
	20170516-jb-59-82-ub-evidence-David-database.txt	Txt
	20180317-jb-119-142-AB-evidence-David-database.txt	Txt
	JB119_110617.raw	Raw

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Publications

Dynamic post-translational modification profiling of <i>Mycobacterium tuberculosis</i>-infected primary macrophages.

Budzik Jonathan M JM Swaney Danielle L DL Jimenez-Morales David D Johnson Jeffrey R JR Garelis Nicholas E NE Repasy Teresa T Roberts Allison W AW Popov Lauren M LM Parry Trevor J TJ Pratt Dexter D Ideker Trey T Krogan Nevan J NJ Cox Jeffery S JS

eLife 20200117

Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translational modifications of proteins during infection with <i>Mycobacterium tuberculosis</i>, a notorious intracellular pathogen. We identified hundreds of dynamically regulated phosphoryl ...[more]

PMID: 31951200

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