Dataset Information

Proteome profiling of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells revealed an unexpected deviation between 2D monolayer and 3D spheroid model cultures.

ABSTRACT: Recently, it has been proposed to employ three-dimensional cultivation of cell in spheroids that contributes more proper physiological conditions in matrix extracellular space architecture and cell morphology accompanied by appropriate intracellular biomechanical organization of cytoskeleton and energy generation apparatus adaptation. In this research we presented the data of proteome survey for cells growing in monolayer and in spheroids that were induced in myogenic way of differentiation and reached terminal stage in seven days. Semi-quantitative profiling of the dynamic changes in proteins abundance depicted strict advantage of spheroid manner cultivation in contrast to monolayer. Cells cultured in spheroid were significantly enriched in proteins of mitochondria biogenesis, respiratory chain proteins, extracellular proteins and cytoskeleton. Most of signal transducers involved in active proliferation and early stages of differentiation were inhibited in spheroid cultures unlike monolayer. The obtained data were broadly consistent with known molecular mechanisms of myogenesis in transcripts level and reflected strong advantage of spheroid cultivation conditions.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Multipotent Stem Cell, Cell Suspension Culture, Cell Culture

SUBMITTER: Arthur Kopylov

LAB HEAD: Pulin Andrey

PROVIDER: PXD015410 | Pride | 2021-11-15

REPOSITORIES: Pride

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Source:

			Action	DRS
	Ging_Even_YT_02.mgf	Mgf
	Ging_Even_YT_02.pride.mgf.gz	Mgf
	Ging_Even_YT_02.raw	Raw
	Ging_Even_YT_02_tpp_psm_export.pep.xml	Pepxml
	Ging_Even_YT_04.mgf	Mgf

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Proteomic and electron microscopy study of myogenic differentiation of alveolar mucosa multipotent mesenchymal stromal cells in three-dimensional culture.

Saburina Irina N IN Kosheleva Nastasia V NV Kopylov Arthur T AT Lipina Tatiana V TV Krasina Marina E ME Zurina Irina M IM Gorkun Anastasiya A AA Girina Svetlana S SS Pulin Andrey A AA Kaysheva Anna L AL Morozov Sergey G SG

Proteomics 20211101 3

Myocyte differentiation is featured by adaptation processes, including mitochondria repopulation and cytoskeleton re-organization. The difference between monolayer and spheroid cultured cells at the proteomic level is uncertain. We cultivated alveolar mucosa multipotent mesenchymal stromal cells in spheroids in a myogenic way for the proper conditioning of ECM architecture and cell morphology, which induced spontaneous myogenic differentiation of cells within spheroids. Electron microscopy analy ...[more]

PMID: 34674377

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