Dataset Information

The novel chemical growth regulator ‘Heatin’ stimulates thermomorphogenesis by inhibiting the Arabidopsis thaliana NIT1-subfamily of nitrilases

ABSTRACT: Temperature passively affects many biological processes. It is therefore challenging to study dedicated temperature signalling pathways orchestrating plant thermomorphogenesis, a suite of elongation growth-based adaptations that enhance leaf cooling capacity. We screened a chemical library for compounds that restored abolished hypocotyl elongation in the pif4-2 deficient mutant background in the model plant Arabidopsis thaliana to identify novel regulators of thermomorphogenesis. The small aromatic compound ‘Heatin’, with 1-aminomethyl-2-naphthol as minimal active moiety, was isolated as potent enhancer of elongation growth. Following a chemical proteomics approach, the NITRILASE1-subfamily auxin biosynthesis enzymes were identified as molecular targets of Heatin. We show that Heatin inhibits NIT1-subfamily enzymatic activity and that accumulation of its substrate indole-3-acetonitrile (IAN) is sufficient for elongation growth in a NIT1-subfamily-dependent manner. Our work assigns a role for NITRILASE1-subfamily in mediating elongation growth. Moreover, Heatin and its functional analogues present novel chemical entities for understanding auxin biology.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Farnusch Kaschani

LAB HEAD: Farnusch Kaschani

PROVIDER: PXD015411 | Pride | 2021-09-08

REPOSITORIES: Pride

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The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases.

van der Woude Lennard L Piotrowski Markus M Klaasse Gruson G Paulus Judith K JK Krahn Daniel D Ninck Sabrina S Kaschani Farnusch F Kaiser Markus M Novák Ondřej O Ljung Karin K Bulder Suzanne S van Verk Marcel M Snoek Basten L BL Fiers Martijn M Martin Nathaniel I NI van der Hoorn Renier A L RAL Robert Stéphanie S Smeekens Sjef S van Zanten Martijn M

The Plant journal : for cell and molecular biology 20210506 6

Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth-based adaptations that enhance leaf-cooling capacity. We screened a chemical library for compounds that restored hypocotyl elongation in the pif4-2-deficient mutant background at warm temperature conditions in Arabidopsis thaliana to identify modulators of thermomorphogen ...[more]

PMID: 33768644

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