Proteomics

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Interactome of AMPK complex study using TAP-MS

ABSTRACT: The adenosine monophosphate-activated protein kinase (AMPK) complex is a key enzyme in regulating cellular energy homeostasis. This complex is well studied and it is known to function in many cellular pathways. However, the interactome of AMPK complex has not yet been systematically established, although protein-protein interaction is critically important for protein function and regulation. Here, we applied tandem-affinity purification coupled with mass spectrometry (TAP-MS) strategy to study the AMPK complex interactome to discover its new functions. We conducted TAP-MS for all the seven AMPK complex subunits. The control TAP-MS group in the dataset includes forty-six control TAP-MS results using non-related baits. HEK293T proteome profiling results were used as background controls in HCIPs analysis. With these analysis, we identified 145 high confident interacting proteins (HCIPs) of AMPK, which allowed us to build an interaction network of AMPK complex.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Zhen Chen  

LAB HEAD: Junjie Chen

PROVIDER: PXD015466 | Pride | 2020-01-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
293T_control_WCE_profile_1.msf Msf
293T_control_WCE_profile_1.raw Raw
293T_control_WCE_profile_10.msf Msf
293T_control_WCE_profile_10.raw Raw
293T_control_WCE_profile_11.msf Msf
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Publications

AMPK Interactome Reveals New Function in Non-homologous End Joining DNA Repair.

Chen Zhen Z   Wang Chao C   Jain Antrix A   Srivastava Mrinal M   Tang Mengfan M   Zhang Huimin H   Feng Xu X   Nie Litong L   Su Dan D   Xiong Yun Y   Jung Sung Yun SY   Qin Jun J   Chen Junjie J  

Molecular & cellular proteomics : MCP 20200103 3

Adenosine monophosphate-activated protein kinase (AMPK) is an obligate heterotrimer that consists of a catalytic subunit (α) and two regulatory subunits (β and γ). AMPK is a key enzyme in the regulation of cellular energy homeostasis. It has been well studied and is known to function in many cellular pathways. However, the interactome of AMPK has not yet been systematically established, although protein-protein interaction is critically important for protein function and regulation. Here, we use  ...[more]

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