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Different osteogenic differentiation behavior of canine mesenchymal stem cells derived from bone marrow and dental pulp: the proteomics profile

ABSTRACT: Although various sources of cMSCs show similar characteristics, they are different in osteogenic potential due to their original cellular sources. Thus, this study was designed to globally explore and analyze the in vitro differentiation potential and behavior of canine bone-marrow derived mesenchymal stem cells (cBM-MSCs) and canine dental pulp stem cells (cDPSCs) toward osteogenic lineage. Global study of an in vitro osteogenic differentiation potential of the isolated cells was performed using proteomic-based analysis through mass spectrometry with dimethyl labelling method at day 7 and 14 post-induction, comparing with undifferentiated cells. The obtained results could be used as a comprehensive data and principal knowledge of the osteogenic differentiation potential of cBM-MSCs and cDPSCs in vitro and the trend of MSC-based tissue engineering for osteogenic regenerative therapy, concentrating on cMSCs application.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Stem Cell, Dental Pulp, Bone Marrow

SUBMITTER: Poorichaya Somparn

LAB HEAD: Chenphop Sawangmake

PROVIDER: PXD015512 | Pride | 2021-09-08

REPOSITORIES: Pride

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Systems biology analysis of osteogenic differentiation behavior by canine mesenchymal stem cells derived from bone marrow and dental pulp.

Nantavisai Sirirat S Pisitkun Trairak T Osathanon Thanaphum T Pavasant Prasit P Kalpravidh Chanin C Dhitavat Sirakarnt S Makjaroen Jiradej J Sawangmake Chenphop C

Scientific reports 20201126 1

Utilization of canine mesenchymal stem cells (cMSCs) for regenerating incorrigible bone diseases has been introduced. However, cMSCs harvested from different sources showed distinct osteogenicity. To clarify this, comparative proteomics-based systems biology analysis was used to analyze osteogenic differentiation behavior by cMSCs harvested from bone marrow and dental pulp. The results illustrated that canine dental pulp stem cells (cDPSCs) contained superior osteogenicity comparing with canine ...[more]

PMID: 33244029

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			Action	DRS
	Bone_Set1_F.msf	Msf
	Bone_Set1_F.pdResult	Other
	Bone_Set1_F1.mgf	Mgf
	Bone_Set1_F1.raw	Raw
	Bone_Set1_F2.mgf	Mgf