Dataset Information

Freshwater benthic cyanobacterial biofilm LC-MSMS

ABSTRACT: In order to understand how Phormidium mats establish, and the role of associated taxa in their development, we collected biofilms over a 19-day growth period during a nitrate-induced bloom event in the Wai-iti River for proteogenomics analysis. At the onset of a late summer bloom, cobbles from the Wai-iti River (Nelson, New Zealand) were removed, cleared of incipient growth with sterile sponges, and placed back into the river. Clearing was gentle as seeding from the pre-existing rock surface is important for bloom establishment [24]. Five pre-cleared cobbles were collected at each of 3 time points to capture the first 3, 6 and 9 days of growth (Table S1). Additional cobbles that contemporaneously developed biofilms were collected at days 12 and 19.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Uncultured Phormidium Sp.

SUBMITTER: Hwee Sze Tee

LAB HEAD: Kim Handley

PROVIDER: PXD015625 | Pride | 2020-05-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	171026171027_KH_pool1_repeat_1in4_120minVSSep2018db.group	Other
	171026171027_KH_pool1_repeat_1in4_120minVSSep2018db.mgf	Mgf
	171026171027_KH_pool1_repeat_1in4_120minVSSep2018dbVs114_ProteinSummary.xlsx	Xlsx
	171026171027_KH_pool2_repeat_1in4_120minVsSep2018db.group	Other
	171026171027_KH_pool2_repeat_1in4_120minVsSep2018db.mgf	Mgf

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Publications

Tools for successful proliferation: diverse strategies of nutrient acquisition by a benthic cyanobacterium.

Tee H S HS Waite D D Payne L L Middleditch M M Wood S S Handley K M KM

The ISME journal 20200518 8

Freshwater cyanobacterial blooms have increased worldwide, channeling organic carbon into these systems, and threatening animal health through the production of cyanotoxins. Both toxic and nontoxic Microcoleus proliferations usually occur when there are moderate concentrations of dissolved inorganic nitrogen, but when phosphorus is scarce. In order to understand how Microcoleus establishes thick biofilms (or mats) on riverbeds under phosphorus-limiting conditions, we collected Microcoleus-domina ...[more]

PMID: 32424245

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