Dataset Information

LC-MSMS of DCC and Neuropilin-1 immunoprecipitated samples from Xenopus Laevis brains

ABSTRACT: During neuronal wiring, extrinsic cues trigger the local translation of specific mRNAs in axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. Here, we analyzed the interactome of endogenous DCC and Neuropilin-1 by analysing immunoprecipitated samples obtained from Xenopus Laevis brains with LC-MSMS. We find that DCC and Neuropilin-1 bind to ribosomal proteins and specific RNA-binding proteins confirming that multiple guidance cue receptors can bind to ribosomes and may use receptor-ribosome coupling to regulate cue-induced local translation in axons.

OTHER RELATED OMICS DATASETS IN: GSE135338

INSTRUMENT(S): Q Exactive

ORGANISM(S): Xenopus Laevis (african Clawed Frog)

TISSUE(S): Brain

SUBMITTER: Toshiaki Shigeoka

LAB HEAD: Christine E. Holt

PROVIDER: PXD015650 | Pride | 2019-12-02

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	M205_3_0718_wa2sNrp1_1.raw	Raw
	M205_3_0718_wa3sDCC_1.raw	Raw
	M205_3_0718_wa3sDCC_2.raw	Raw
	M205_3_0718_wa3sDCC_3.raw	Raw
	M205_3_0718_wa3sDCC_4.raw	Raw

Items per page:

1 - 5 of 47

Publications

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Koppers Max M Cagnetta Roberta R Shigeoka Toshiaki T Wunderlich Lucia Cs LC Vallejo-Ramirez Pedro P Qiaojin Lin Julie J Zhao Sixian S Jakobs Maximilian Ah MA Dwivedy Asha A Minett Michael S MS Bellon Anaïs A Kaminski Clemens F CF Harris William A WA Flanagan John G JG Holt Christine E CE

eLife 20191120

Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. We report that receptor-ribosome coupling is employed by multiple guidance cue receptors and this interaction is mRNA-dependent. We find that different receptors associ ...[more]

PMID: 31746735

Dataset Information

LC-MSMS of DCC and Neuropilin-1 immunoprecipitated samples from Xenopus Laevis brains

Dataset's files

Publications

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Similar Datasets

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons
2019-11-29 | GSE135338 | GEO

Subcellular expression profiling of the growth cones of retinal ganglion cells (RGC)
2010-11-23 | E-GEOD-25166 | biostudies-arrayexpress

Subcellular expression profiling of the growth cones of retinal ganglion cells (RGC)
2010-11-23 | GSE25166 | GEO

The dynamic translatome of retinal ganglion cell axons during assembly and maintenance of the mouse visual system
2016-06-16 | E-GEOD-79352 | biostudies-arrayexpress

Axonal ER tubules control local translation via P180/RRBP1-mediated ribosome interactions
2024-04-29 | GSE262262 | GEO

miR-182 regulates Slit2-mediated axon guidance by modulating the local translation of a specific mRNA
2017-01-23 | GSE86883 | GEO

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons
| PRJNA558460 | ENA

Transcription profiling of mouse rat cortical axons from un-injured and injured animals isolated after 13 days in culture
2009-01-14 | E-GEOD-11730 | biostudies-arrayexpress

mRNAs in un-injured and injured rat cortical axons isolated after 13 days in culture
2009-01-01 | GSE11730 | GEO

The dynamic translatome of retinal ganglion cell axons during assembly and maintenance of the mouse visual system
2016-06-16 | GSE79352 | GEO