Dataset Information

Site mapping of PGK1 O-GlcNAcylation

ABSTRACT: To identify the site(s) of O-GlcNAcylation on PGK1, we transiently co-expressed Flag-tagged PGK1 and OGT in HEK293T cells. After immunoprecipitation using anti-Flag M2 beads and in-gel trypsin digestion, resulted peptides were subjected to mass spectrometry analysis

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Hao Nie

LAB HEAD: Wen Yi

PROVIDER: PXD015659 | Pride | 2019-11-06

REPOSITORIES: Pride

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Publications

O-GlcNAcylation of PGK1 coordinates glycolysis and TCA cycle to promote tumor growth.

Nie Hao H Ju Haixing H Fan Jiayi J Shi Xiaoliu X Cheng Yaxian Y Cang Xiaohui X Zheng Zhiguo Z Duan Xiaotao X Yi Wen W

Nature communications 20200107 1

Many cancer cells display enhanced glycolysis and suppressed mitochondrial metabolism. This phenomenon, known as the Warburg effect, is critical for tumor development. However, how cancer cells coordinate glucose metabolism through glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle is largely unknown. We demonstrate here that phosphoglycerate kinase 1 (PGK1), the first ATP-producing enzyme in glycolysis, is reversibly and dynamically modified with O-linked N-acetylglucosamine (O-Glc ...[more]

PMID: 31911580

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			Action	DRS
	PGK1-1.mgf	Mgf
	PGK1-1.mzXML	Mzxml
	PGK1-1.raw	Raw
	PGK1-11.raw	Raw
	PGK1-2.mgf	Mgf