Dataset Information

Hydrogen utilization by Methylocystis sp. strain SC2 expands the known metabolic versatility of type IIa methanotrophs

ABSTRACT: The objective of this study was to assess whether Methylocystis sp. strain SC2, as a representative for Methylocystis spp., can utilize hydrogen to optimize the biomass yield by mixed utilization of CH4 and H2, rather than CH4 as the sole source of energy. Thus, we aimed to show that, in the presence of H2, CH4 will primarily be used for synthesis of cell carbon and increased biomass/protein yield. In particular, we intended to explore those CH4/O2 ratios, which maximize the effect of hydrogen addition on the biomass yield and proteome reconstruction of strain SC2. To achieve our goals, we combined hydrogen-based growth experiments with our recently optimized proteomics workflow.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bacteria Methylocystis Sp. Sc2

SUBMITTER: Anna Hakobyan

LAB HEAD: PD Dr. Werner Liesack

PROVIDER: PXD015700 | Pride | 2020-06-03

REPOSITORIES: Pride

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			Action	DRS
	Re-SC2-22-10.msf	Msf
	Re-SC2-22-10.mzid.gz	Mzid
	Re-SC2-22-10.mzid_Re-SC2-22-10.MGF	Mzid
	Re-SC2-22-10.mzid_Re-SC2-22-10.pride.mgf.gz	Mzid
	Re-SC2-22-10.pride.mztab.gz	Mztab

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Hydrogen utilization by Methylocystis sp. strain SC2 expands the known metabolic versatility of type IIa methanotrophs.

Hakobyan Anna A Zhu Jing J Glatter Timo T Paczia Nicole N Liesack Werner W

Metabolic engineering 20200530

Methane, a non-expensive natural substrate, is used by Methylocystis spp. as a sole source of carbon and energy. Here, we assessed whether Methylocystis sp. strain SC2 is able to also utilize hydrogen as an energy source. The addition of 2% H2 to the culture headspace had the most significant positive effect on the growth yield under CH4 (6%) and O2 (3%) limited conditions. The SC2 biomass yield doubled from 6.41 (±0.52) to 13.82 (±0.69) mg cell dry weight per mm ...[more]

PMID: 32479801

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