Dataset Information

Phosphoproteomic Characterization of Signaling Networks Resulting from Activation of Chemokine Receptor CCR2

ABSTRACT: Leukocyte recruitment, a universal feature of tissue inflammation, is regulated by the interactions of chemokines with their G protein-coupled receptors. Activation of CC chemokine receptor 2 (CCR2) by its cognate chemokine ligands, including CCL2, plays a central role in recruitment of monocytes in several inflammatory diseases. In this study, we used phosphoproteomics to enable unbiased characterization of the signaling network resulting from CCL2 activation of CCR2. Using data-independent acquisition (DIA) mass spectrometry, both the proteome and phosphoproteome were quantified for FlpIn-HEK293T cells stably expressing CCR2, at six time points after activation with CCL2, in comparison with untreated cells. Differential expression analysis identified 699 significantly regulated phosphorylation sites located on 441 proteins. As expected, many of these proteins participate in canonical signal transduction pathways and the regulation of actin cytoskeleton dynamics, including numerous guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). In addition, we identified regulated phosphorylation sites in numerous proteins that exert functions in the nucleus, including several constituents of the nuclear pore complex. This study provides an unprecedented level of detail about CCR2 signalling and identifies potential novel targets for regulation of CCR2 function.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Early Embryonic Cell

SUBMITTER: Cheng Huang

LAB HEAD: Martin J Stone

PROVIDER: PXD015845 | Pride | 2020-04-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20170107_213723_HEK_CCR2_Phos_Timecourse_Pep_Report.xls	Xls
	20170107_234001_HEK_CCR2_Phos_Timecourse_SpectronautAnalysis.sne	Other
	20170120_230822_HEK_CCR2_Total_Timecourse_SpectronautAnalysis.sne	Other
	20170121_181539_HEK_CCR2_Total_Timecourse_Report_ProteinQuant.xls	Xls
	P1CHH_201601229_HEK_Phos_DIA_0min_r1_375-1275.htrms	Other

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Phosphoproteomic characterization of the signaling network resulting from activation of the chemokine receptor CCR2.

Huang Cheng C Foster Simon R SR Shah Anup D AD Kleifeld Oded O Canals Meritxell M Schittenhelm Ralf B RB Stone Martin J MJ

The Journal of biological chemistry 20200402 19

Leukocyte recruitment is a universal feature of tissue inflammation and regulated by the interactions of chemokines with their G protein-coupled receptors. Activation of CC chemokine receptor 2 (CCR2) by its cognate chemokine ligands, including CC chemokine ligand 2 (CCL2), plays a central role in recruitment of monocytes in several inflammatory diseases. In this study, we used phosphoproteomics to conduct an unbiased characterization of the signaling network resulting from CCL2 activation of CC ...[more]

PMID: 32241914

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