Proteomics

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Subcellular quantitative proteomic analysis of herpes simplex virus type 1 infected HEK 293T cells


ABSTRACT: Herpes simplex virus type-1 (HSV-1) is wide-spread dsDNA virus that establishes life-long latency and causes diverse severity of symptoms. In this study, HEK 293T cells with low Toll-like receptor (TLR) and Stimulator of interferon genes protein (STING) expression was infected with HSV-1 and subjected to quantitative proteomic analysis. By using a subcellular fractionation strategy and high-performance mass spectrometry, a total of 5,982 host proteins were quantified, of which 484 proteins were differentially regulated. Bioinformatics analysis indicated that multiple signaling pathways were involved in HSV-1 infection.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Leike Zhang  

LAB HEAD: Liangjie Wang

PROVIDER: PXD015887 | Pride | 2019-12-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20140311_AC1-10__FDR.xlsx Xlsx
20140311_AC1.wiff Wiff
20140311_AC1.wiff.scan Wiff
20140311_AC10.wiff Wiff
20140311_AC10.wiff.scan Wiff
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Publications

A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells.

Wan Weiwei W   Wang Liangjie L   Chen Xi X   Zhu Shenglin S   Shang Weijuan W   Xiao Gengfu G   Zhang Lei-Ke LK  

Molecules (Basel, Switzerland) 20191120 23


Herpes simplex virus type 1 (HSV-1) is widespread double-stranded DNA (dsDNA) virus that establishes life-long latency and causes diverse severe symptoms. The mechanisms of HSV-1 infection and HSV-1's interactions with various host cells have been studied and reviewed extensively. Type I interferons were secreted by host cells upon HSV infection and play a vital role in controlling virus proliferation. A few studies, however, have focused on HSV-1 infection without the presence of interferon (IF  ...[more]

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