Dataset Information

Analysis of the N-Glycoforms of a Chinese Yam (Dioscorea opposita Thunb.) Glycoprotein 30CYGPAnalysis of the N-Glycoforms of a Chinese Yam (Dioscorea opposita Thunb.) Glycoprotein 30CYGP

ABSTRACT: Chinese yam (Dioscorea opposita Thumb.) is a highly nutritional perennial herbaceous rhizome. N-glycoforms from Chinese yam glycoprotein were analyzed, and the major glycoprotein in yam be identificated.

INSTRUMENT(S): LTQ

ORGANISM(S): Dioscorea Oppositifolia

TISSUE(S): Root

SUBMITTER: li cheng

LAB HEAD: Cheng li

PROVIDER: PXD015888 | Pride | 2019-10-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	ESI-MSprofilesoftheN-glycansreleasedfromChineseyamproteins.raw	Raw
	MascotSearchResult.zip	Other
	ProfilesofPMP-labeledN-glycansweredetectedbyESI-MS.raw	Raw
	Quantitativeanalysisof30CYGPN-glycansbyonlineLC-MSMS.RAW	Raw
	Result.zip	Other

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Publications

Analysis of the N-Glycoforms and Immunoactivity of Chinese Yam (Dioscorea opposita Thunb.) Glycoprotein 30CYGP.

Li Cheng C Lu Yu Y Chen Xiaoyi X Yang Meifang M Zou Zihua Z Han Jianli J Gao Xi X Tang Rui R Wang Chengjian C Huang Linjuan L Wang Zhongfu Z

Journal of proteome research 20191101 1

The major protein in Chinese yam (Dioscorea opposita Thunb.) glycoprotein, 30CYGP, exhibits strong immunomodulatory activities. Research has identified the sequence and structure of 30CYGP; however, 30CYGP N-glycoform composition and immunoactivity remain unknown. We isolated and purified 30CYGP from Chinese yam and used that material to release the N-glycans contained within. The N-glycans were labeled with 1-phenyl-3-methyl-5-pyrazolone and analyzed via ESI-MS and o ...[more]

PMID: 31647243

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Project description:The leaf of Chinese cabbage is the major place of photosynthesis, the mutation of leaf may directly affect the rate of plant growth and development and the formation of leafy head, and ultimately influence the yield and quality of Chinese cabbage. We identified a developmentally retarded mutant (drm) exhibiting stable inheritance, which was derived from Chinese cabbage DH line âFTâ using a combination of isolated microspore culture and radiation treatment (60Co Î³-rays). The drm exhibited slow growth and development at the seedling and heading stages, leading to the production of a tiny, leafy head, as well as chlorophyll-deficient leaves, especially in seedlings. Genetic analysis indicated that the phenotype of drm was controlled by a single recessive nuclear gene. Compared with wild-type line âFTâ, the drmâs chlorophyll content was significantly reduced and its chloroplast structure was abnormal. Moreover, the photosynthetic efficiency and chlorophyll fluorescence parameters were significantly decreased. The changes in leaf color, combined with these altered physiological characters may influence the growth and development of plant, ultimately resulting in the developmentally retarded phenotype of drm. To further understand the molecular regulatory mechanisms of phenotypic differences between âFTâ and drm, comparative transcriptome analysis were performed using RNA-Seq, a total of 338 differentially expressed genes (DEGs) were detected between âFTâ and drm. According to GO and KEGG pathway analysis, a number of DEGs which involved in the chlorophyll degradation and photosynthesis were identified, such as chlorophyllase and ribulose-1,5-bisphosphate carboxylase/oxygenase. In addition, the expression patterns of 12 DEGs, including three chlorophyll degradation- and photosynthesis-related genes and nine randomly selected genes, were confirmed by qRT-PCR. Numerous single nucleotide polymorphisms were also identified, providing a valuable resource for research and molecular marker-assistant breeding in Chinese cabbage. These results contribute to our understanding of the molecular regulatory mechanisms underlying growth and development and lay the foundation for future genetic and functional genomics studies in Chinese cabbage. The RNA from the third true leaves (day 15 to day 24 after the appearance of the third true leaves) of a developmentally retarded mutant (drm) and its wild type âFTâ in Chinese cabbage were sequenced by RNA-Seq, in triplicate.

Dataset Information

Analysis of the N-Glycoforms of a Chinese Yam (Dioscorea opposita Thunb.) Glycoprotein 30CYGPAnalysis of the N-Glycoforms of a Chinese Yam (Dioscorea opposita Thunb.) Glycoprotein 30CYGP

Dataset's files

Publications

Analysis of the <i>N</i>-Glycoforms and Immunoactivity of Chinese Yam (<i>Dioscorea opposita</i> Thunb.) Glycoprotein 30CYGP.

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