Dataset Information

Quantitative phosphoproteomics analysis uncovers the regulatory networks of ZNF322A in lung cancer cells

ABSTRACT: Cys2His2 (C2H2)-type zinc finger (ZNF) is one of the most common DNA-binding motif and plays a major role as transcription factors, which encoded by 2% of human genes. C2H2 type ZNFs are reported as proteins of great versatility, such as regulation of differentiation, development, metabolism, cell survival, apoptosis, and autophagy. To uncover ZNF322A-mediated functional network, we applied phosphopeptide enrichment and isobaric labeling strategies with MS-based proteomics, and identified 2754 phosphorylation sites on 976 phosphoproteins.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Lung Cancer

SUBMITTER: Chantal Hoi Yin Cheung

LAB HEAD: Hsueh-Fen Juan, Ph.D.

PROVIDER: PXD015936 | Pride | 2021-09-08

REPOSITORIES: Pride

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ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer.

Cheung Chantal Hoi Yin CHY Hsu Chia-Lang CL Lin Tsai-Yu TY Chen Wei-Ting WT Wang Yi-Ching YC Huang Hsuan-Cheng HC Juan Hsueh-Fen HF

Journal of biomedical science 20200623 1

<h4>Background</h4>ZNF322A is an oncogenic transcription factor that belongs to the Cys2His2-type zinc-finger protein family. Accumulating evidence suggests that ZNF322A may contribute to the tumorigenesis of lung cancer, however, the ZNF322A-mediated downstream signaling pathways remain unknown.<h4>Methods</h4>To uncover ZNF322A-mediated functional network, we applied phosphopeptide enrichment and isobaric labeling strategies with mass spectrometry-based proteomics using A549 lung cancer cells, ...[more]

PMID: 32576196

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