Proteomics

Dataset Information

0

Chemical phosphoproteomics reveals kinase network topologies associated to genotypes and phenotypes of cancer cells.

ABSTRACT: We compare the in vitro specificity profiles of several kinase inhibitors with their effects on cellular phosphoproteomes. For this aim, we developed an algorithm which utilizes information on kinase inhibitor selectivity to determine the most probable kinase(s) acting upstream of phosphorylation sites present in phosphoproteomics data obtained from cancer cells treated with kinase inhibitors.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Testis, Myeloblast, Colorectal Cell Line, Peripheral Blood, Epithelial Cell, Breast

DISEASE(S): Acute Myeloid Leukemia,Colorectal Cancer,Embryonal Testis Carcinoma,Breast Cancer

SUBMITTER: Maruan Hijazi  

LAB HEAD: Pedro Cutillas

PROVIDER: PXD015943 | Pride | 2020-01-29

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
27aa5b1532242ea4___b1790p076_NTERA_PP1_CX_r1.raw.-1.mgf Mgf
27aa5b1532242ea4___b1790p076_NTERA_PP1_CX_r2.raw.-1.mgf Mgf
27aa5b1532242ea4___b1790p076_NTERA_PP1_DMSO_r1.raw.-1.mgf Mgf
27aa5b1532242ea4___b1790p076_NTERA_PP1_DMSO_r2.raw.-1.mgf Mgf
27aa5b1532242ea4___b1790p076_NTERA_PP1_Go_r1.raw.-1.mgf Mgf
Items per page:
1 - 5 of 2538
altmetric image

Publications

Reconstructing kinase network topologies from phosphoproteomics data reveals cancer-associated rewiring.

Hijazi Maruan M   Smith Ryan R   Rajeeve Vinothini V   Bessant Conrad C   Cutillas Pedro R PR  

Nature biotechnology 20200120 4

Understanding how oncogenic mutations rewire regulatory-protein networks is important for rationalizing the mechanisms of oncogenesis and for individualizing anticancer treatments. We report a chemical phosphoproteomics method to elucidate the topology of kinase-signaling networks in mammalian cells. We identified >6,000 protein phosphorylation sites that can be used to infer >1,500 kinase-kinase interactions and devised algorithms that can reconstruct kinase network topologies from these phosph  ...[more]

Similar Datasets

Proteomics Chemical phosphoproteomics reveals kinase network topologies associated to genotypes and phenotypes of cancer cells.
2022-10-22 | MSV000090571 | MassIVE
Proteomics Community detection from empirical kinase networks identifies new members of signalling pathways
2023-06-07 | PXD026039 | Pride
Proteomics Targeting LSD1 rewires kinase networks and primes leukaemia cells for kinase inhibitor treatment
2022-04-25 | PXD018603 | Pride
Proteomics Elucidating the role of the kinase activity of endothelial cell focal adhesion kinase in angiocrine signalling and tumour growth
2021-11-02 | PXD019728 | Pride
Proteomics Kinase activity ranking (KAR) quantifies the contribution of protein kinases to the regulation of cell viability
2017-07-06 | PXD005073 | Pride
Proteomics Computational analysis of cholangiocarcinoma phosphoproteomes identifies patient-specific drug targets
2023-03-11 | PXD027329 | Pride
Proteomics BRAF mutations disrupt the hypothalamo-pituitary axis leading to hypopituitarism in mouse and humans
2020-11-17 | PXD018190 | Pride
Proteomics An increase in oxidative stress downstream of mTORC1 but not AKT causes a proliferative defect in cancer cells with acquired resistance to PI3K/mTOR inhibitors
2018-04-09 | PXD003594 | Pride
Proteomics Signaling network diversification during differentiation sensitizes AML cells to PAK and MEK inhibitors
2020-04-15 | PXD005978 | Pride
Proteomics Evaluation of of kinase acitivity profiling using chemical proteomics
2016-10-21 | MSV000080272 | MassIVE