Dataset Information

A comprehensive CHO SWATH-MS spectral library for accurate quantitative profiling of 10k proteins

ABSTRACT: A comprehensive Chinese hamster ovary (CHO)-cell specific spectral library, containing extensive information of high-quality spectral ions covering more than 10k CHO cell proteins, was constructed to provide confident and reproducible protein identification and quantification in data-independent SWATH-MS analysis. The applicability of CHO spectral library was tested in the analyses of different CHO samples including whole cell lysate, harvested cell culture fluids, and downstream processing samples. The portability of CHO spectral library was also demonstrated in the processing and analyses of SWATH-MS data sets collected from multiple LC-MS instrumental setups and various CHO cell lines.

INSTRUMENT(S): TripleTOF 5600+, TripleTOF 6600

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Epithelial Cell

SUBMITTER: Kae Hwan Sim

LAB HEAD: Xuezhi Bi

PROVIDER: PXD016047 | Pride | 2020-07-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CHOK1_HM_bRPLC_01.group	Other
	CHOK1_HM_bRPLC_01.wiff	Wiff
	CHOK1_HM_bRPLC_01.wiff.scan	Wiff
	CHOK1_HM_bRPLC_02.group	Other
	CHOK1_HM_bRPLC_02.wiff	Wiff

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A comprehensive CHO SWATH-MS spectral library for robust quantitative profiling of 10,000 proteins.

Sim Kae Hwan KH Liu Lillian Chia-Yi LC Tan Hwee Tong HT Tan Kelly K Ng Daniel D Zhang Wei W Yang Yuansheng Y Tate Stephen S Bi Xuezhi X

Scientific data 20200811 1

Sequential window acquisition of all theoretical fragment-ion spectra (SWATH) is a data-independent acquisition (DIA) strategy that requires a specific spectral library to generate unbiased and consistent quantitative data matrices of all peptides. SWATH-MS is a promising approach for in-depth proteomic profiling of Chinese hamster Ovary (CHO) cell lines, improving mechanistic understanding of process optimization, and real-time monitoring of process parameters in biologics R&D and manufacturing ...[more]

PMID: 32782267

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