Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion
ORGANISM(S): Xenopus Laevis (african Clawed Frog)
SUBMITTER: Joao Paulo
LAB HEAD: Joao A. Paulo
PROVIDER: PXD016164 | Pride | 2022-09-02
REPOSITORIES: Pride
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Cell chemical biology 20210107 4
Deubiquitylating enzymes (DUBs) counteract ubiquitylation to control stability or activity of substrates. Identification of DUB substrates is challenging because multiple DUBs can act on the same substrate, thwarting genetic approaches. Here, we circumvent redundancy by chemically inhibiting multiple DUBs simultaneously in Xenopus egg extract. We used quantitative mass spectrometry to identify proteins whose ubiquitylation or stability is altered by broad DUB inhibition, and confirmed their DUB- ...[more]