Proteomics

Dataset Information

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Metaproteomics on the digestive tract from Gammarus fossarum, an environmentally relevant sentinel.

ABSTRACT: Presentation of a new data treatment methodology for investigating the full range of biomasses and metabolic activities of the holobiont components from the digestive tract of Gammarus fossarum. Two tissues, intestine and hepatopancreatic caecum from individual animals were compared. Three animals have been analyzed in technical duplicates.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Gammarus Fossarum

SUBMITTER: Jean ARMENGAUD  

LAB HEAD: Jean Armengaud

PROVIDER: PXD016248 | Pride | 2021-09-09

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
DB_DDG_metaP-round3.fasta Fasta
F409941.dat Other
F409941_p0.0027852.mzid.gz Mzid
F409941_p0.0027852.pride.mztab.gz Mztab
F409942.dat Other
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Publications

Combining proteogenomics and metaproteomics for deep taxonomic and functional characterization of microbiomes from a non-sequenced host.

Gouveia Duarte D   Pible Olivier O   Culotta Karen K   Jouffret Virginie V   Geffard Olivier O   Chaumot Arnaud A   Degli-Esposti Davide D   Armengaud Jean J  

NPJ biofilms and microbiomes 20200605 1

Metaproteomics of gut microbiomes from animal hosts lacking a reference genome is challenging. Here we describe a strategy combining high-resolution metaproteomics and host RNA sequencing (RNA-seq) with generalist database searching to survey the digestive tract of Gammarus fossarum, a small crustacean used as a sentinel species in ecotoxicology. This approach provides a deep insight into the full range of biomasses and metabolic activities of the holobiont components, and differentiates between  ...[more]

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