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Targeted quantification of flg22-responsive BSU1 phosphorylation sites

ABSTRACT: To confirm the flg22-induced BSU1 phosphorylation site, we performed targeted quantification by PRM analysis with SILIA IP-MS sample. The isotopes switched in replicate experiments.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Shoot

SUBMITTER: Shouling Xu

LAB HEAD: Shouling Xu

PROVIDER: PXD016257 | Pride | 2023-03-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	BSU1skyline.sky	Other
	BSU1skyline.sky.view	Other
	BSU1skyline.skyd	Other
	Q20171010_02.pep.xml	Pepxml
	Q20171010_02.raw	Raw

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1 - 5 of 7

Publications

Deconvoluting signals downstream of growth and immune receptor kinases by phosphocodes of the BSU1 family phosphatases.

Park Chan Ho CH Bi Yang Y Youn Ji-Hyun JH Kim So-Hee SH Kim Jung-Gun JG Xu Nicole Y NY Shrestha Ruben R Burlingame Alma L AL Xu Shou-Ling SL Mudgett Mary Beth MB Kim Seong-Ki SK Kim Tae-Wuk TW Wang Zhi-Yong ZY

Nature plants 20220613 6

Hundreds of leucine-rich repeat receptor kinases (LRR-RKs) have evolved to control diverse processes of growth, development and immunity in plants, but the mechanisms that link LRR-RKs to distinct cellular responses are not understood. Here we show that two LRR-RKs, the brassinosteroid hormone receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) and the flagellin receptor FLAGELLIN SENSING 2 (FLS2), regulate downstream glycogen synthase kinase 3 (GSK3) and mitogen-activated protein (MAP) kinases, respe ...[more]

PMID: 35697730

Similar Datasets

Identification of flg22-responsive BSU1 phosphorylation sites

Project description:To investigate flg22-induced posttranslational modification of BSU1 family proteins, we performed metabolic Stable Isotope Labeling In Arabidopsis followed by Immuno-Precipitation and Mass Spectrometry (SILIA IP-MS) analysis, with the isotopes switched in replicate experiments.

2023-03-10 | PXD016283 | Pride

Expression analysis of Arabidopsis mpk11 mutant treated with flg22

Project description:Arabidopsis MPK11 is activated and plays a role in the flg22 sensing. Mitogen-activated protein kinases (MAPKs) mediate cellular signal transduction during stress responses, as well as diverse growth and developmental steps in eukaryotes. Pathogen infection or treatment with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPKs, MPK3, MPK4 and MPK6. Several stresses, including flg22 treatment, are known to increase MPK11 expression but activation of MPK11 has not been shown. Here, we show that MPK11 activity can indeed be increased through flg22 elicitation. Expression profiling using a small-scale microarray for defense-related genes revealed that cinnamyl alcohol dehyrogenase 5 (CAD5) requires MPK11 for full flg22-induced expression. An mpk11 mutant showed increased flg22-mediated growth inhibition but no altered susceptibility to Pseudomonas syringae, Botrytis cinerea or Alternaria brassicicola. A 24 DNA microarray study using toral RNA from an Arabidopsis mutant (mpk11 SALK049352) as well as wild type treated with water or flg22.

2012-05-02 | E-GEOD-33648 | biostudies-arrayexpress

RNA-seq of Arabidopsis thaliana plants treated with putrescine, spermine, flg22 and combinations

Project description:RNA-seq of Arabidopsis thaliana plants (Col-0) treated with putrescine (100 µM), spermine (100 µM), flg22 (1 µM) and combinations (putrescine + flg22; spermine + flg22)

2022-10-20 | E-MTAB-11820 | biostudies-arrayexpress

flg22 time course-Gene profiling upon biotic stress treatment: Gene profiling in microRNA mutants

Project description:rs06-06_mirna - flg22 time course - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - 14-day old seedlings (Col-0 and fls2 KO) treated with 10uM of flg22 (flagellin derived peptide) over a one hour timecourse. Keywords: time course,treated vs untreated comparison 7 dye-swap - CATMA arrays

2008-06-01 | E-GEOD-10418 | biostudies-arrayexpress

flg22 treatment & mutants-Gene profiling upon biotic stress treatment. Gene profiling in microRNA mutants.

Project description:rs06-06_mirna - flg22 treatment & mutants - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? 14-day old seedlings treated with flg22 (flagellin- derived peptide). Keywords: time course,total transcriptome knowledge 7 dye-swap - CATMA arrays

2008-06-01 | E-GEOD-10383 | biostudies-arrayexpress

Response to different N-acyl homoserine lactones and N-acyl homoserine lactones-pretreated plants to secondary challenge with flg22.

Project description:In order to gain coherent insights into plant responses we performed transcriptional analysis of Arabidopsis seedling after treatment with three different AHLs: N-hexanoyl-L-homoserine lactone (C6-HSL), N-3-oxo-decanoyl-L-homoserine lactone (oxo-C10-HSL), and N-3-oxo-tetradecanoyl-L-homoserine lactone (oxo-C14-HSL). Furthermore, we analyzed the transcriptome of oxo-C14-HSL pretreated plants after a secondary challenge with 100 nM flg22 for 2 and 24 hours after treatment. Gene expression in Arabidopsis thaliana seedlings were measured after a 3-days-pretreatment with 6 ÂµM of three different N-acyl homoserine lactones in comparison to plants treated with the coresponding volume of acetone (solvent control). Plants pretreated with oxo-C14-HSL were in addition treated with 100nM of flg22 to induced defense response. Three independent experiments were performed (replicates).

2014-11-26 | E-GEOD-52979 | biostudies-arrayexpress

Identification of early flg22 responsive genes in Arabidopsis seedlings

Project description:The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis seedlings that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs). Arabidopsis seedlings were treated with 2 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.

2010-03-18 | E-GEOD-17382 | biostudies-arrayexpress

Identification of early flg22 responsive genes in Arabidopsis mesophyll cells

Project description:The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs). Arabidopsis mesophyll protoplasts were treated with 100 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.

2010-03-18 | E-GEOD-16472 | biostudies-arrayexpress

flg22 treatment & mutants 2-Gene profiling upon biotic stress treatment. Gene profiling in microRNA mutants.

Project description:rs06-06_mirna - flg22 treatment & mutants 2 - Time course : What are the genes (incuding microRNA precursors) that are differentially regulated upon flg22 treatment? miRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of microRNA mutants? siRNA : What are the genes (including miRNA precursors) that are differentially regulated in a set of siRNA mutants? - Col-0, dcl4-1, rdr6-1 were grown for 12-day on MS solid medium ,seedlings were then transferred in MS liquid medium 2 days after and treated with 10uM flagellin active or inactive. Samples were harvested at 30 min after treatment. Keywords: treated vs untreated comparison 3 dye-swap - CATMA arrays

2008-06-01 | E-GEOD-10355 | biostudies-arrayexpress

Quantitative O-GlcNAc analysis between wild type and sec-5 using SILIA (F2 R1 on QE-HF)

Project description:O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. Plants have close OGT homologs, SECRET AGENT (SEC) and SPINDLY (SPY). In vitro, SEC has shown O-GlcNAc activity. Recently, a surprising discovery in wheat revealed an atypical TaOGT(TaOGT1) with no structural similarity to SEC and SPY enzyme (Fan et al. 2021). TaOGT1 was found to O-GlcNAcylate TaGRP2. In Arabidopsis, approximately 34 unannotated or uncharacterized proteins share similarity with TaOGT1, leading to questions about whether SEC is the primary contributor to O-GlcNAcylation in plants. Here we use LWAC enrichment and SILIA labeling, quantifying at both MS1. Our findings reveal a significant reduction in O-GlcNAc levels in the sec mutant, indicating SEC’s critical role in mediating O-GlcNAcylation.

2024-05-22 | PXD044831 | Pride

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