Proteomics

Dataset Information

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Arabidopsis SME interactome reveals spliceosome components


ABSTRACT: Alternative splicing (AS) can produce multiple transcripts with different exon-intron structures from a single pre-mRNA. Pre-mRNA splicing is catalyzed by a dynamic macromolecular ribonucleoprotein (RNP) complex termed the spliceosome. The spliceosome consists of several small nuclear ribonucleoproteins (snRNPs) that bind uridine-rich small nuclear RNA (snRNA). In U1, U2, U4 and U5 snRNPs, snRNA interacts with the conserved Smith antigen (Sm) proteins via a bipartite Sm sequence motif. In eukaryotes, seven Sm proteins (B, D1/2/3, E, F and G) form a heptameric ring-shaped complex surrounding the snRNA. Here, we performed a tandem affinity purification using SMEB as a bait in Arabidopsis cell suspension cultures. At least 45 known/hypothesized and potential novel spliceosome components were identified in Arabidopsis.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Patrick Willems  

LAB HEAD: Frank Van Breusegem

PROVIDER: PXD016607 | Pride | 2023-03-03

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MaxQuant_txt_folder_and_proteinDB.zip Other
V21921_TAP_Dominique_09-09-14-G1-L3_rerun.raw Raw
V21923_TAP_Dominique_09-09-14-G1-L4_rerun.raw Raw
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