NanoFreeProteomics (NFP) Compared to Conventional nanoLC based proteomics
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Cell Suspension Culture, Permanent Cell Line Cell
DISEASE(S): Colon Cancer
SUBMITTER: 
Ben Orsburn
LAB HEAD: Benjamin Orsburn
PROVIDER: PXD016704 | Pride | 2020-02-25
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| BCO_TMT_Vanquish_F1.raw | Raw | |||
| BCO_TMT_Vanquish_F10.raw | Raw | |||
| BCO_TMT_Vanquish_F11.raw | Raw | |||
| BCO_TMT_Vanquish_F12.raw | Raw | |||
| BCO_TMT_Vanquish_F13.raw | Raw |
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Project description:Data analyzed within Procedure 2 in Kantnerova et al. 202X in Nature Protocols. Procedure 2 describes the stable isotope analysis of the model analyte nitrate (NO3-) by applying the flow injection using an HPLC system with an autosampler.
The nitrate data were collected using a Thermo Scientific Orbitrap Exploris 240 Isotope Solutions.
Sample = USGS32, Reference = USGS35 (nitrate isotopic reference materials from the United States Geological Survey), 50 uM nitrate in methanol.
Isotopocule data collected "with M0" base peak.
RAW files were processed using the IsoX software (version 2022) using the isotopologs.tsv files as input.
There are three datasets with the following prefixes (to be removed before re-analysis using the IsoX software and the R scripts):
"01_" - USGS32 vs USGS35, optimal ion source conditions, 13 alternating injections of USGS35 and USGS32 + 2 blank methanol measurements
"02_" - USGS32 vs USGS35, non-optimal ion-source conditions, 13 alternating injections of USGS35 and USGS32 + 2 blank methanol measurements
"03_" - USGS32 vs USGS35, alternating injections of USGS35 and USGS32 extended to a total of 315 injections
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