Proteomics

Dataset Information

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Complexome profiling of mitochondrial protein complexes in single and double MIC26 and MIC27 knockout cells

ABSTRACT: Here we analysed the impact of two subunits of the mitochondrial contact site and cristae organizing system (MICOS) complex, MIC26 and MIC27, on the assembly and stability of mitochondrial complexes of the oxidative phosphorylation system (OXPHOS).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Culture

SUBMITTER: Ilka Wittig  

LAB HEAD: Andreas Reichert

PROVIDER: PXD016733 | Pride | 2020-07-28

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
Complexome_profiling_OXPHOS.xlsx Xlsx
Cutting_matrix.jpg Other
MaxQuant_Output.zip Other
P18_016_MICOS_HAP1WT_01.raw Raw
P18_016_MICOS_HAP1WT_02.raw Raw
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Publications

Homologous apolipoproteins of MICOS complex, MIC26 and MIC27, show an antagonistic regulation of their protein levels, making it difficult to deduce their individual functions using a single gene deletion. We obtained single and double knockout (DKO) human cells of <i>MIC26</i> and <i>MIC27</i> and found that DKO show more concentric onion-like cristae with loss of CJs than any single deletion indicating overlapping roles in formation of CJs. Using a combination of complexome profiling, STED nan  ...[more]

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